                         SEQUENCE LISTING

<110>  Commonwealth Scientific and Industrial Research 
       Organisation
       The Australian National University
       Grains Research and Development Corporation
 
<120>  Methods of producing carbamoyl phosphate and urea

<130>  512000

<150>  US 61/498,395
<151>  2011-06-17

<160>  49    

<170>  PatentIn version 3.5

<210>  1
<211>  314
<212>  PRT
<213>  Pyrococcus furiosus

<400>  1

Met Gly Lys Arg Val Val Ile Ala Leu Gly Gly Asn Ala Leu Gln Gln 
1               5                   10                  15      


Arg Gly Gln Lys Gly Ser Tyr Glu Glu Met Met Asp Asn Val Arg Lys 
            20                  25                  30          


Thr Ala Arg Gln Ile Ala Glu Ile Ile Ala Arg Gly Tyr Glu Val Val 
        35                  40                  45              


Ile Thr His Gly Asn Gly Pro Gln Val Gly Ser Leu Leu Leu His Met 
    50                  55                  60                  


Asp Ala Gly Gln Ala Thr Tyr Gly Ile Pro Ala Gln Pro Met Asp Val 
65                  70                  75                  80  


Ala Gly Ala Met Ser Gln Gly Trp Ile Gly Tyr Met Ile Gln Gln Ala 
                85                  90                  95      


Leu Lys Asn Glu Leu Arg Lys Arg Gly Met Glu Lys Lys Val Val Thr 
            100                 105                 110         


Ile Ile Thr Gln Thr Ile Val Asp Lys Asn Asp Pro Ala Phe Gln Asn 
        115                 120                 125             


Pro Thr Lys Pro Val Gly Pro Phe Tyr Asp Glu Glu Thr Ala Lys Arg 
    130                 135                 140                 


Leu Ala Arg Glu Lys Gly Trp Ile Val Lys Glu Asp Ser Gly Arg Gly 
145                 150                 155                 160 


Trp Arg Arg Val Val Pro Ser Pro Asp Pro Lys Gly His Val Glu Ala 
                165                 170                 175     


Glu Thr Ile Lys Lys Leu Val Glu Arg Gly Val Ile Val Ile Ala Ser 
            180                 185                 190         


Gly Gly Gly Gly Val Pro Val Ile Leu Glu Asp Gly Glu Ile Lys Gly 
        195                 200                 205             


Val Glu Ala Val Ile Asp Lys Asp Leu Ala Gly Glu Lys Leu Ala Glu 
    210                 215                 220                 


Glu Val Asn Ala Asp Ile Phe Met Ile Leu Thr Asp Val Asn Gly Ala 
225                 230                 235                 240 


Ala Leu Tyr Tyr Gly Thr Glu Lys Glu Gln Trp Leu Arg Glu Val Lys 
                245                 250                 255     


Val Glu Glu Leu Arg Lys Tyr Tyr Glu Glu Gly His Phe Lys Ala Gly 
            260                 265                 270         


Ser Met Gly Pro Lys Val Leu Ala Ala Ile Arg Phe Ile Glu Trp Gly 
        275                 280                 285             


Gly Glu Arg Ala Ile Ile Ala His Leu Glu Lys Ala Val Glu Ala Leu 
    290                 295                 300                 


Glu Gly Lys Thr Gly Thr Gln Val Leu Pro 
305                 310                 


<210>  2
<211>  314
<212>  PRT
<213>  Pyrococcus horikoshii

<400>  2

Met Pro Glu Arg Val Val Ile Ala Leu Gly Gly Asn Ala Leu Gln Gln 
1               5                   10                  15      


Arg Gly Gln Lys Gly Thr Tyr Asp Glu Met Met Glu Asn Val Arg Lys 
            20                  25                  30          


Thr Ala Lys Gln Ile Ala Glu Ile Ile Ala Arg Gly Tyr Glu Val Val 
        35                  40                  45              


Ile Thr His Gly Asn Gly Pro Gln Val Gly Thr Ile Leu Leu His Met 
    50                  55                  60                  


Asp Ala Gly Gln Ser Leu His Gly Ile Pro Ala Gln Pro Met Asp Val 
65                  70                  75                  80  


Ala Gly Ala Met Ser Gln Gly Trp Ile Gly Tyr Met Ile Gln Gln Ala 
                85                  90                  95      


Leu Arg Asn Glu Leu Arg Lys Arg Gly Ile Glu Lys Glu Val Val Thr 
            100                 105                 110         


Ile Ile Thr Gln Thr Ile Val Asp Lys Lys Asp Pro Ala Phe Gln Asn 
        115                 120                 125             


Pro Thr Lys Pro Val Gly Pro Phe Tyr Asp Glu Lys Thr Ala Lys Lys 
    130                 135                 140                 


Leu Ala Lys Glu Lys Gly Trp Val Val Lys Glu Asp Ala Gly Arg Gly 
145                 150                 155                 160 


Trp Arg Arg Val Val Pro Ser Pro Asp Pro Lys Gly His Val Glu Ala 
                165                 170                 175     


Glu Thr Ile Arg Arg Leu Val Glu Ser Gly Ile Ile Val Ile Ala Ser 
            180                 185                 190         


Gly Gly Gly Gly Val Pro Val Ile Glu Glu Asn Gly Glu Ile Lys Gly 
        195                 200                 205             


Val Glu Ala Val Ile Asp Lys Asp Leu Ala Gly Glu Lys Leu Ala Glu 
    210                 215                 220                 


Glu Val Asn Ala Asp Ile Leu Met Ile Leu Thr Asp Val Asn Gly Ala 
225                 230                 235                 240 


Ala Leu Tyr Tyr Gly Thr Glu Lys Glu Thr Trp Leu Arg Asn Val Lys 
                245                 250                 255     


Val Glu Glu Leu Glu Lys Tyr Tyr Gln Glu Gly His Phe Lys Ala Gly 
            260                 265                 270         


Ser Met Gly Pro Lys Val Leu Ala Ala Ile Arg Phe Ile Lys Asn Gly 
        275                 280                 285             


Gly Lys Arg Ala Ile Ile Ala His Leu Glu Lys Ala Val Glu Ala Leu 
    290                 295                 300                 


Glu Gly Lys Thr Gly Thr Gln Val Thr Pro 
305                 310                 


<210>  3
<211>  314
<212>  PRT
<213>  Pyrococcus abyssi

<400>  3

Met Pro Glu Arg Val Val Ile Ala Leu Gly Gly Asn Ala Leu Gln Gln 
1               5                   10                  15      


Arg Gly Gln Lys Gly Thr Tyr Glu Glu Met Met Glu Asn Val Lys Lys 
            20                  25                  30          


Thr Ala Lys Gln Ile Ala Glu Ile Ile Ala Arg Gly Tyr Glu Val Val 
        35                  40                  45              


Ile Thr His Gly Asn Gly Pro Gln Val Gly Thr Ile Leu Leu His Met 
    50                  55                  60                  


Asp Ala Gly Gln Ser Leu His Gly Ile Pro Ala Gln Pro Met Asp Val 
65                  70                  75                  80  


Ala Gly Ala Met Ser Gln Gly Trp Ile Gly Tyr Met Ile Gln Gln Ala 
                85                  90                  95      


Leu Arg Asn Glu Leu Arg Lys Arg Gly Ile Glu Arg Glu Val Val Thr 
            100                 105                 110         


Ile Val Thr Gln Thr Ile Val Asp Lys Asp Asp Pro Ala Phe Glu Asn 
        115                 120                 125             


Pro Thr Lys Pro Val Gly Pro Phe Tyr Asp Glu Glu Thr Ala Lys Lys 
    130                 135                 140                 


Leu Ala Lys Glu Lys Gly Trp Val Val Lys Glu Asp Ala Gly Arg Gly 
145                 150                 155                 160 


Trp Arg Arg Val Val Pro Ser Pro Asp Pro Lys Arg His Val Glu Ala 
                165                 170                 175     


Glu Thr Ile Lys Lys Leu Val Glu Asp Gly Val Ile Val Ile Ala Ser 
            180                 185                 190         


Gly Gly Gly Gly Val Pro Val Ile Glu Glu Asp Gly Glu Ile Lys Gly 
        195                 200                 205             


Val Glu Ala Val Ile Asp Lys Asp Leu Ala Gly Glu Arg Leu Ala Glu 
    210                 215                 220                 


Glu Val Asn Ala Asp Ile Leu Met Ile Leu Thr Asp Val Asn Gly Ala 
225                 230                 235                 240 


Ala Leu Tyr Tyr Gly Thr Glu Lys Glu Thr Trp Leu Arg Glu Val Lys 
                245                 250                 255     


Val Asp Glu Met Glu Arg Tyr Tyr Gln Glu Gly His Phe Lys Ala Gly 
            260                 265                 270         


Ser Met Gly Pro Lys Val Leu Ala Ala Ile Arg Phe Val Lys Asn Gly 
        275                 280                 285             


Gly Lys Arg Ala Ile Ile Ala His Leu Glu Lys Ala Val Glu Ala Leu 
    290                 295                 300                 


Glu Gly Lys Thr Gly Thr Gln Val Ile Pro 
305                 310                 


<210>  4
<211>  314
<212>  PRT
<213>  Thermococcus sp.

<400>  4

Met Lys Arg Val Val Ile Ala Leu Gly Gly Asn Ala Ile Leu Gln Arg 
1               5                   10                  15      


Gly Gln Lys Gly Thr Tyr Glu Glu Gln Met Glu Asn Val Arg Lys Thr 
            20                  25                  30          


Ala Arg Gln Ile Ala Asp Ile Ile Glu Arg Gly Tyr Glu Val Val Ile 
        35                  40                  45              


Thr His Gly Asn Gly Pro Gln Val Gly Ala Leu Leu Leu His Met Asp 
    50                  55                  60                  


Val Gly Gln Gln Val Tyr Gly Ile Pro Ala Gln Pro Met Asp Val Ala 
65                  70                  75                  80  


Gly Ala Met Thr Gln Gly Gln Ile Gly Tyr Met Ile Gln Gln Ala Leu 
                85                  90                  95      


Ile Asn Glu Leu Arg Ala Arg Gly Ile Glu Lys Pro Val Ala Thr Ile 
            100                 105                 110         


Val Thr Gln Thr Leu Val Asp Lys Asn Asp Pro Ala Phe Gln Asn Pro 
        115                 120                 125             


Ser Lys Pro Val Gly Pro Phe Tyr Asp Glu Glu Thr Ala Lys Lys Leu 
    130                 135                 140                 


Ala Arg Glu Lys Gly Trp Val Val Val Glu Asp Ser Gly Arg Gly Trp 
145                 150                 155                 160 


Arg Arg Val Val Pro Ser Pro Asp Pro Lys Gly His Val Glu Ala Pro 
                165                 170                 175     


Val Ile Gln Asp Leu Val Glu Lys Gly Phe Ile Val Ile Ala Ser Gly 
            180                 185                 190         


Gly Gly Gly Val Pro Val Val Glu Glu Asp Gly Arg Leu Lys Gly Val 
        195                 200                 205             


Glu Ala Val Ile Asp Lys Asp Leu Ala Gly Glu Arg Leu Ala Glu Glu 
    210                 215                 220                 


Val Glu Ala Asp Ile Phe Met Ile Leu Thr Asp Val Asn Gly Ala Ala 
225                 230                 235                 240 


Ile Asn Phe Gly Lys Pro Asp Glu Arg Trp Leu Gly Lys Val Thr Val 
                245                 250                 255     


Glu Glu Leu Lys Lys Tyr Tyr Ala Glu Gly His Phe Lys Lys Gly Ser 
            260                 265                 270         


Met Gly Pro Lys Val Leu Ala Val Ile Arg Phe Val Glu Trp Gly Gly 
        275                 280                 285             


Glu Arg Gly Ile Ile Ala Ser Leu Asp Lys Ala Val Glu Ala Leu Glu 
    290                 295                 300                 


Gly Lys Thr Gly Thr Gln Val Ile Lys Gly 
305                 310                 


<210>  5
<211>  316
<212>  PRT
<213>  Thermococcus gammatolerans

<400>  5

Met Ile Leu Met Lys Arg Val Val Ile Ala Leu Gly Gly Asn Ala Ile 
1               5                   10                  15      


Leu Gln Arg Gly Gln Arg Gly Thr Tyr Glu Glu Gln Met Glu Asn Val 
            20                  25                  30          


Arg Lys Thr Ala Lys Gln Ile Ala Asp Ile Ile Glu Arg Gly Tyr Glu 
        35                  40                  45              


Val Val Ile Thr His Gly Asn Gly Pro Gln Val Gly Ala Leu Leu Leu 
    50                  55                  60                  


His Met Asp Ala Gly Gln Gln Leu Tyr Gly Ile Pro Ala Gln Pro Met 
65                  70                  75                  80  


Asp Val Ala Gly Ala Met Thr Gln Gly Gln Ile Gly Tyr Met Ile Gly 
                85                  90                  95      


Gln Ala Leu Ile Asn Glu Leu Arg Lys Arg Gly Ile Asp Arg Pro Val 
            100                 105                 110         


Ala Thr Ile Val Thr Gln Thr Ile Val Asp Lys Asn Asp Pro Ala Phe 
        115                 120                 125             


Lys Asn Pro Ser Lys Pro Val Gly Pro Phe Tyr Asp Glu Glu Thr Ala 
    130                 135                 140                 


Lys Lys Leu Ala Lys Glu Lys Gly Trp Val Val Ile Glu Asp Ala Gly 
145                 150                 155                 160 


Arg Gly Trp Arg Arg Val Val Pro Ser Pro Asp Pro Lys Gly His Val 
                165                 170                 175     


Glu Ala Pro Val Ile Gln Asp Leu Val Glu Lys Gly Phe Ile Val Ile 
            180                 185                 190         


Ala Ser Gly Gly Gly Gly Val Pro Val Ile Glu Glu Asn Gly Glu Leu 
        195                 200                 205             


Lys Gly Val Glu Ala Val Ile Asp Lys Asp Leu Ala Gly Glu Lys Leu 
    210                 215                 220                 


Ala Glu Glu Val Lys Ala Asp Ile Phe Met Ile Leu Thr Asp Val Asn 
225                 230                 235                 240 


Gly Ala Ala Ile Asn Phe Gly Lys Pro Asp Glu Arg Trp Leu Glu Arg 
                245                 250                 255     


Val Thr Val Glu Glu Leu Arg Lys Tyr Tyr Glu Glu Gly His Phe Lys 
            260                 265                 270         


Arg Gly Ser Met Gly Pro Lys Val Leu Ala Val Ile Arg Phe Leu Glu 
        275                 280                 285             


Trp Gly Gly Glu Arg Ala Ile Ile Ala Ser Leu Asp Arg Ala Val Glu 
    290                 295                 300                 


Ala Leu Glu Gly Lys Thr Gly Thr Gln Val Phe Pro 
305                 310                 315     


<210>  6
<211>  315
<212>  PRT
<213>  Thermococcus kodakarensis

<400>  6

Met Lys Arg Val Val Ile Ala Leu Gly Gly Asn Ala Ile Leu Gln Arg 
1               5                   10                  15      


Gly Gln Lys Gly Thr Tyr Glu Glu Gln Met Glu Asn Val Arg Arg Thr 
            20                  25                  30          


Ala Lys Gln Ile Ala Asp Ile Ile Leu Asp Gly Asp Tyr Glu Val Val 
        35                  40                  45              


Ile Thr His Gly Asn Gly Pro Gln Val Gly Ala Leu Leu Leu His Met 
    50                  55                  60                  


Asp Ala Gly Gln Gln Val Tyr Gly Ile Pro Ala Gln Pro Met Asp Val 
65                  70                  75                  80  


Ala Gly Ala Met Thr Gln Gly Gln Ile Gly Tyr Met Ile Gly Gln Ala 
                85                  90                  95      


Leu Ile Asn Glu Leu Arg Lys Arg Gly Val Glu Lys Pro Val Ala Thr 
            100                 105                 110         


Ile Val Thr Gln Thr Ile Val Asp Lys Asn Asp Pro Ala Phe Gln Asn 
        115                 120                 125             


Pro Ser Lys Pro Val Gly Pro Phe Tyr Asp Glu Glu Thr Ala Lys Lys 
    130                 135                 140                 


Leu Ala Lys Glu Lys Gly Trp Thr Val Ile Glu Asp Ala Gly Arg Gly 
145                 150                 155                 160 


Trp Arg Arg Val Val Pro Ser Pro Asp Pro Lys Gly His Val Glu Ala 
                165                 170                 175     


Pro Val Ile Val Asp Leu Val Glu Lys Gly Phe Ile Val Ile Ala Ser 
            180                 185                 190         


Gly Gly Gly Gly Val Pro Val Ile Glu Glu Asn Gly Glu Leu Lys Gly 
        195                 200                 205             


Val Glu Ala Val Ile Asp Lys Asp Leu Ala Gly Glu Lys Leu Ala Glu 
    210                 215                 220                 


Glu Val Lys Ala Asp Ile Phe Met Ile Leu Thr Asp Val Asn Gly Ala 
225                 230                 235                 240 


Ala Ile Asn Tyr Gly Lys Pro Asp Glu Lys Trp Leu Gly Lys Val Thr 
                245                 250                 255     


Val Asp Glu Leu Lys Arg Tyr Tyr Lys Glu Gly His Phe Lys Lys Gly 
            260                 265                 270         


Ser Met Gly Pro Lys Val Leu Ala Ala Ile Arg Phe Val Glu Trp Gly 
        275                 280                 285             


Gly Glu Arg Ala Val Ile Ala Ser Leu Asp Arg Ala Val Glu Ala Leu 
    290                 295                 300                 


Glu Gly Lys Thr Gly Thr Gln Val Val Arg Glu 
305                 310                 315 


<210>  7
<211>  315
<212>  PRT
<213>  Thermococcus onnurineus

<400>  7

Met Lys Arg Val Val Ile Ala Leu Gly Gly Asn Ala Ile Leu Gln Arg 
1               5                   10                  15      


Gly Gln Lys Gly Thr Tyr Glu Glu Gln Met Thr Asn Val Met Lys Thr 
            20                  25                  30          


Ala Lys Gln Ile Val Asp Ile Ile Leu Asp Gly Asp Tyr Glu Val Val 
        35                  40                  45              


Ile Thr His Gly Asn Gly Pro Gln Ile Gly Ala Leu Leu Leu His Met 
    50                  55                  60                  


Asp Ala Gly Gln Gln Ile His Gly Ile Pro Ala Gln Pro Met Asp Val 
65                  70                  75                  80  


Ala Gly Ala Met Thr Gln Gly Gln Ile Gly Tyr Met Ile Gln Gln Ala 
                85                  90                  95      


Ile Arg Asn Glu Leu Lys Arg Arg Gly Val Glu Arg Pro Val Ala Thr 
            100                 105                 110         


Ile Val Thr Gln Thr Leu Val Asp Lys Asn Asp Pro Ala Phe Gln Asn 
        115                 120                 125             


Pro Ser Lys Pro Val Gly Pro Phe Tyr Asp Glu Glu Thr Ala Lys Arg 
    130                 135                 140                 


Leu Ala Lys Glu Lys Gly Trp Thr Val Ile Glu Asp Ser Gly Arg Gly 
145                 150                 155                 160 


Trp Arg Arg Val Val Pro Ser Pro Asp Pro Ile Gly His Val Glu Thr 
                165                 170                 175     


Pro Val Ile Gln Asp Leu Val Glu Lys Gly Phe Ile Val Ile Ala Ser 
            180                 185                 190         


Gly Gly Gly Gly Val Pro Val Ile Glu Glu Asp Gly Met Leu Lys Gly 
        195                 200                 205             


Val Glu Ala Val Ile Asp Lys Asp Leu Ala Gly Glu Lys Leu Ala Glu 
    210                 215                 220                 


Glu Val Asn Ala Asp Ile Phe Met Ile Leu Thr Asp Val Asn Gly Ala 
225                 230                 235                 240 


Ala Ile Asn Tyr Gly Lys Pro Asp Glu Lys Trp Leu Gly Arg Val Thr 
                245                 250                 255     


Val Glu Glu Leu Lys Arg Tyr Tyr Asn Glu Gly His Phe Lys Lys Gly 
            260                 265                 270         


Ser Met Gly Pro Lys Val Leu Ala Ala Ile Arg Phe Val Glu Trp Gly 
        275                 280                 285             


Gly Glu Arg Ala Val Ile Ala Ala Leu Asp Lys Ala Val Glu Ala Leu 
    290                 295                 300                 


Glu Gly Lys Thr Gly Thr Gln Val Ile Lys Gly 
305                 310                 315 


<210>  8
<211>  316
<212>  PRT
<213>  Thermococcus barophilus

<400>  8

Met Arg Lys Arg Val Val Ile Ala Leu Gly Gly Asn Ala Ile Leu Gln 
1               5                   10                  15      


Arg Gly Gln Lys Gly Thr Tyr Asp Glu Gln Met Glu Asn Val Lys Lys 
            20                  25                  30          


Thr Ala Lys Gln Ile Val Asp Ile Ile Leu Asn Asn Asp Tyr Glu Val 
        35                  40                  45              


Val Ile Thr His Gly Asn Gly Pro Gln Val Gly Ala Leu Leu Leu His 
    50                  55                  60                  


Met Asp Ala Gly Gln Gln Leu Tyr Gly Ile Pro Ala Gln Pro Met Asp 
65                  70                  75                  80  


Val Ala Gly Ala Met Thr Gln Gly Gln Ile Gly Tyr Met Ile Gln Gln 
                85                  90                  95      


Ala Ile Thr Asn Glu Leu Lys Arg Arg Gly Ile Tyr Lys Pro Val Ala 
            100                 105                 110         


Thr Ile Val Thr Gln Val Leu Val Asp Lys Asn Asp Pro Ala Phe Gln 
        115                 120                 125             


Asn Pro Ser Lys Pro Val Gly Pro Phe Tyr Asp Glu Glu Thr Ala Lys 
    130                 135                 140                 


Arg Leu Ala Lys Glu Lys Glu Trp Val Val Val Glu Asp Ala Gly Arg 
145                 150                 155                 160 


Gly Trp Arg Arg Val Val Pro Ser Pro Asp Pro Lys Asp Ile Ile Glu 
                165                 170                 175     


Lys Asp Ile Ile Arg Asp Leu Val Glu Lys Gly Phe Ile Val Ile Ala 
            180                 185                 190         


Ser Gly Gly Gly Gly Ile Pro Val Ile Glu Glu Asn Gly Gln Leu Lys 
        195                 200                 205             


Gly Val Glu Ala Val Ile Asp Lys Asp Leu Ala Gly Glu Lys Leu Ala 
    210                 215                 220                 


Glu Val Val Asn Ala Asp Ile Phe Met Ile Leu Thr Asp Val Asn Gly 
225                 230                 235                 240 


Ala Ala Ile Asn Tyr Gly Lys Pro Asn Glu Arg Trp Leu His Lys Val 
                245                 250                 255     


Ala Val Asp Glu Leu Arg Lys Tyr Tyr Glu Glu Gly His Phe Lys Lys 
            260                 265                 270         


Gly Ser Met Gly Pro Lys Val Leu Ala Ala Ile Arg Phe Val Glu Trp 
        275                 280                 285             


Gly Gly Glu Arg Ala Val Ile Ala Ala Leu Asp Lys Ala Val Asp Ala 
    290                 295                 300                 


Leu Glu Gly Arg Thr Gly Thr Gln Val Ile Lys Met 
305                 310                 315     


<210>  9
<211>  315
<212>  PRT
<213>  Thermococcus sibiricus

<400>  9

Met Arg Lys Arg Val Val Ile Ala Leu Gly Gly Asn Ala Ile Leu Gln 
1               5                   10                  15      


Arg Gly Gln Lys Gly Thr Tyr Glu Glu Gln Met Glu Asn Val Arg Lys 
            20                  25                  30          


Thr Ala Arg Gln Ile Val Asp Ile Ile Leu Asp Asn Glu Tyr Glu Val 
        35                  40                  45              


Val Ile Thr His Gly Asn Gly Pro Gln Val Gly Ala Leu Leu Leu Gln 
    50                  55                  60                  


Gln Asp Ala Gly Glu His Val His Gly Ile Pro Ala Gln Pro Met Asp 
65                  70                  75                  80  


Val Cys Gly Ala Met Ser Gln Gly Gln Ile Gly Tyr Met Ile Gln Gln 
                85                  90                  95      


Ala Ile Met Asn Glu Leu Arg Arg Arg Gly Val Glu Arg Pro Val Ala 
            100                 105                 110         


Thr Ile Val Thr Gln Thr Ile Val Asp Lys Asn Asp Pro Ala Phe Gln 
        115                 120                 125             


His Pro Ser Lys Pro Val Gly Pro Phe Tyr Ser Glu Glu Thr Ala Lys 
    130                 135                 140                 


Lys Leu Ala Lys Glu Lys Gly Trp Val Val Ile Glu Asp Ala Gly Arg 
145                 150                 155                 160 


Gly Trp Arg Arg Val Val Pro Ser Pro Asp Pro Lys Gly His Val Glu 
                165                 170                 175     


Ala Pro Ile Ile Gln Asp Leu Val Glu Lys Glu Phe Ile Val Ile Ser 
            180                 185                 190         


Ser Gly Gly Gly Gly Ile Pro Val Val Glu Glu Asn Gly Glu Leu Lys 
        195                 200                 205             


Gly Val Glu Ala Val Ile Asp Lys Asp Leu Ala Gly Glu Arg Leu Ala 
    210                 215                 220                 


Glu Glu Val Asn Ala Asp Ile Phe Met Ile Leu Thr Asp Val Asn Gly 
225                 230                 235                 240 


Ala Ala Ile Asn Tyr Gly Arg Pro Asn Glu Lys Trp Leu Glu Lys Val 
                245                 250                 255     


Thr Leu Gly Glu Ile Lys Arg Tyr Tyr Glu Glu Gly His Phe Lys Lys 
            260                 265                 270         


Gly Ser Met Gly Pro Lys Val Leu Ala Ala Ile Arg Phe Ile Glu Trp 
        275                 280                 285             


Gly Gly Glu Arg Ala Ile Ile Ala Ala Leu Asp Lys Ala Val Glu Ala 
    290                 295                 300                 


Leu Glu Gly Lys Thr Gly Thr Gln Ile Thr Arg 
305                 310                 315 


<210>  10
<211>  942
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized Pfu CK open reading frame

<400>  10
atgggtaaac gtgttgttat tgccctgggt ggtaatgcac tgcagcagcg tggtcagaaa       60

ggtagctatg aagaaatgat ggataatgtg cgtaaaaccg cacgtcagat tgcagaaatc      120

attgcccgtg gttatgaagt tgttattacc catggtaatg gtccgcaggt tggtagcctg      180

ctgctgcaca tggatgcagg tcaggcaacc tatggtattc cggcacagcc gatggatgtt      240

gccggtgcaa tgagccaggg ttggattggt tatatgattc agcaggccct gaaaaatgaa      300

ctgcgtaaac gtggcatgga aaaaaaagtg gtgaccatta ttacccagac cattgtggat      360

aaaaatgatc cggcatttca gaatccgact aaaccggttg gtccgtttta tgatgaagaa      420

accgcaaaac gtctggcacg tgaaaaaggt tggattgtga aagaagatag cggtcgcggt      480

tggcgtcgtg ttgttccgtc accggatccg aaaggtcatg ttgaagccga aaccattaag      540

aaactggtgg aacgtggtgt tattgttatt gcaagcggtg gtggtggtgt tccggttatt      600

ctggaagatg gcgaaatcaa aggtgttgaa gccgtgattg ataaagatct ggcaggcgaa      660

aaactggcag aagaagtgaa cgccgatatc tttatgattc tgaccgatgt taatggtgca      720

gcactgtatt atggcaccga aaaagaacag tggctgcgtg aagttaaagt tgaagaactg      780

cgcaaatact atgaagaagg ccattttaaa gcaggtagca tgggtccgaa agttctggca      840

gccattcgtt ttattgaatg gggtggtgaa cgtgcaatta ttgcccatct ggaaaaagca      900

gttgaagcac tggaaggtaa aaccggcacc caggttctgc cg                         942


<210>  11
<211>  945
<212>  DNA
<213>  Pyrococcus furiosus

<400>  11
atgggtaaga gggtagtgat tgcacttgga ggtaacgctc ttcagcagcg aggtcaaaag       60

ggaagttatg aggagatgat ggataacgtt cgcaagaccg ctaggcaaat tgccgaaatt      120

atagcgagag ggtatgaagt tgttatcact catggaaatg gtcctcaagt tggaagtctt      180

ctccttcata tggatgctgg gcaggcaact tatggaattc ccgcccaacc aatggatgtg      240

gctggtgcaa tgagtcaggg atggattggt tatatgattc agcaggcttt gaagaacgag      300

ctgaggaaga ggggcatgga aaagaaagtt gttacaataa ttacccaaac aattgttgat      360

aagaatgatc cagcattcca aaaccccaca aagccagtgg ggccatttta cgatgaagag      420

actgcaaaaa ggttagccag agaaaaggga tggatagtta aagaggattc tggtagaggt      480

tggagaagag tagttccttc tccagatccc aaagggcacg ttgaggcaga gacgattaaa      540

aagttagtgg aaaggggagt tatagttatt gcaagtggtg gaggaggagt tcctgtaata      600

cttgaagacg gggaaataaa gggtgttgag gccgtcatcg acaaggatct ggcaggagaa      660

aagcttgctg aggaagtaaa tgctgacata ttcatgattc ttacagatgt taacggcgct      720

gcattatact atggaacgga aaaggagcag tggttaaggg aagttaaggt cgaagagctt      780

aggaagtact atgaagaggg ccactttaag gcgggaagca tggggccaaa ggttctagcg      840

gctataaggt tcatcgagtg gggtggagag agggccataa tagctcacct agaaaaggca      900

gttgaggctc tcgaagggaa gactggtact caagttctcc cttaa                      945


<210>  12
<211>  945
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Pyrococcus 
       horikoshii carbamate kinase

<400>  12
atgccggaac gtgttgttat tgcactgggt ggtaatgcac tgcagcagcg tggtcagaaa       60

ggcacctatg atgaaatgat ggaaaatgtt cgtaaaaccg ccaaacaaat cgccgaaatt      120

attgcacgtg gttatgaagt tgttatcacc catggtaatg gtccgcaggt tggcaccatt      180

ctgctgcata tggatgcagg tcagagcctg catggtattc cggcacagcc gatggatgtt      240

gccggtgcaa tgagccaggg ttggattggt tatatgattc agcaggcact gcgtaatgaa      300

ctgcgtaaac gtggtattga aaaagaagtg gtgaccatta ttacccagac catcgtggat      360

aaaaaagatc cggcatttca gaatccgacc aaaccggtgg gtccgtttta tgatgagaaa      420

accgcaaaaa aactggccaa agaaaaaggt tgggtggtta aagaagatgc cggtcgcggt      480

tggcgtcgtg ttgttccgag tccggatccg aaaggtcatg ttgaagcaga aaccattcgt      540

cgtctggttg aaagcggtat tattgtgatt gcaagcggtg gtggtggcgt tccggttatt      600

gaagaaaatg gtgaaatcaa aggtgtggaa gccgtgattg ataaagatct ggcaggcgag      660

aaactggcag aagaagttaa cgcagatatt ctgatgattc tgaccgatgt taatggtgca      720

gcactgtatt atggcaccga aaaagaaacc tggctgcgca atgttaaagt tgaggaactg      780

gaaaaatact accaagaggg tcattttaaa gcaggtagca tgggtccgaa agttctggca      840

gcaattcgtt ttatcaaaaa tggtggtaaa cgtgccatta tcgcccatct ggaaaaagca      900

gttgaagcac tggaaggtaa aaccggcacc caggttaccc cgtaa                      945


<210>  13
<211>  945
<212>  DNA
<213>  Pyrococcus horikoshii

<400>  13
atgcccgaga gagttgttat cgccctggga ggaaacgcac tccagcagag agggcaaaaa       60

gggacttatg atgagatgat ggagaacgta aggaaaacgg ctaaacagat agctgagata      120

attgcgaggg gttacgaagt cgttataact cacggtaacg gacctcaggt tgggacgatt      180

ctccttcata tggatgcggg acaatccctt catggaatac cagcccaacc catggatgtt      240

gccggggcga tgagccaagg atggataggt tacatgattc aacaagcgtt gaggaatgaa      300

ctcaggaaaa gagggataga aaaggaagtt gtcactataa taactcaaac gatagttgac      360

aaaaaagatc cagcatttca aaatccaacg aagcctgtag gcccattcta tgatgaaaaa      420

actgcaaaga aacttgcaaa ggagaaagga tgggttgtca aggaagatgc aggaagggga      480

tggagaaggg ttgtcccaag cccagatccc aagggacacg tcgaggctga aacgataaga      540

agactcgtcg aaagtgggat tatagttata gcaagtgggg gaggaggagt ccccgtaatt      600

gaagagaatg gagaaataaa aggcgttgaa gccgtgatag acaaagatct agctggcgaa      660

aaattggccg aggaggttaa tgcagatata cttatgatac taacggatgt caacggggcc      720

gccctatact atggaacgga gaaggaaact tggcttagaa acgttaaggt ggaagaactg      780

gagaagtact accaagaggg gcactttaag gctggaagca tgggtcctaa agttcttgca      840

gcaataaggt tcattaaaaa tggaggaaaa agagcaataa tagctcacct tgagaaagct      900

gttgaagccc ttgaaggaaa gacaggaacc caggtgactc cctaa                      945


<210>  14
<211>  945
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Pyrococcus abyssi 
       carbamate kinase

<400>  14
atgccggaac gtgttgttat tgcactgggt ggtaatgcac tgcagcagcg tggtcagaaa       60

ggcacctatg aagaaatgat ggaaaacgtg aaaaaaaccg ccaaacaaat cgccgaaatt      120

attgcacgtg gttatgaagt tgttatcacc catggtaatg gtccgcaggt tggcaccatt      180

ctgctgcata tggatgcagg tcagagcctg catggtattc cggcacagcc gatggatgtt      240

gccggtgcaa tgagccaggg ttggattggt tatatgattc agcaggcact gcgtaatgaa      300

ctgcgtaaac gtggtattga acgtgaagtt gtgaccattg ttacccagac cattgtggat      360

aaagatgatc cggcatttga aaatccgacc aaaccggtgg gtccgtttta tgatgaagaa      420

accgcaaaaa aactggccaa agaaaaaggt tgggtggtta aagaagatgc cggtcgcggt      480

tggcgtcgtg ttgttccgag tccggatccg aaacgtcatg ttgaagcaga aaccatcaaa      540

aaactggttg aagatggcgt tattgttatc gcaagcggtg gtggtggcgt tccggttatt      600

gaagaggatg gtgaaatcaa aggtgttgaa gccgtgattg ataaagatct ggcaggcgaa      660

cgtctggcag aagaagttaa cgcagatatt ctgatgattc tgaccgatgt taatggtgca      720

gcactgtatt atggcaccga aaaagaaacc tggctgcgtg aagttaaagt ggatgaaatg      780

gaacgctatt atcaagaggg tcattttaaa gcaggtagca tgggtccgaa agttctggca      840

gcaattcgtt ttgttaaaaa tggtggtaaa cgtgccatta tcgcccatct ggaaaaagca      900

gttgaagcac tggaaggtaa aaccggcacc caggttattc cgtaa                      945


<210>  15
<211>  945
<212>  DNA
<213>  Pyrococcus abyssi

<400>  15
atgccggaga gagtcgtcat agccctcgga ggaaacgccc ttcagcagag gggtcaaaag       60

ggaacgtacg aggagatgat ggagaacgtt aagaaaactg caaagcagat agctgaaatt      120

atcgcgaggg gttacgaagt ggttataacc cacggaaatg gccctcaggt agggacgatc      180

ctccttcaca tggacgctgg ccaatcactt cacggaattc cagctcagcc catggacgtt      240

gctggggcaa tgagccaggg atggataggt tacatgatac agcaggcact gaggaacgag      300

cttaggaaga gggggataga gagggaggta gttacgatag ttacccagac aatagtagat      360

aaggacgatc ctgccttcga gaacccaact aaaccagttg gcccgttcta cgacgaggaa      420

actgcaaaga aactagctaa ggaaaaggga tgggtagtga aggaagacgc tggaagggga      480

tggaggaggg tagttccaag cccagatcca aagaggcacg tagaggctga gaccataaag      540

aagttagttg aggatggcgt tatagttata gcgagcggcg ggggaggagt gccggtaata      600

gaggaggatg gagagataaa aggggtcgaa gcggtaatag acaaggatct agcgggagag      660

aggttagctg aggaggttaa cgctgacata ctgatgatct taacggatgt aaacggggca      720

gcactctact acggaaccga gaaggagacc tggcttagag aagttaaggt ggacgagatg      780

gagaggtact atcaagaagg gcacttcaag gccggaagca tggggccaaa ggttttagct      840

gctataaggt tcgtgaagaa cggaggaaag agggcaataa ttgctcacct tgagaaagct      900

gttgaagccc tggaaggaaa gaccgggaca caggttattc cctga                      945


<210>  16
<211>  948
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Thermococcus sp. 
       carbamate kinase

<400>  16
atgaaacgtg ttgttattgc cctgggtggt aatgcaattc tgcagcgtgg tcagaaaggc       60

acctatgaag aacaaatggc caatgttatg aaaaccgcca aacaaatcgt ggatattatt      120

ctggatggcg attatgaagt ggtgattacc catggtaatg gtccgcaggt tggtgcactg      180

ctgctgcata tggatgcagg tcaggcaacc catggcattc cggcacagcc gatggatgtt      240

gccggtgcaa tgacccaggg tcagattggt tacatgattc agcaggcaat tcgcaatgaa      300

ctgaaacgtc gtggtattga tcgtccggtt gccaccattg ttacccagac cattgttgat      360

aaaaacgatc cggcatttca gcatccgagc aaaccggttg gtccgtttta tgatgaagaa      420

accgcaaaaa aactggccga agaaaaaggt tgggttgttg ttgaagatag cggtcgtggt      480

tggcgtcgtg ttgttccgag tccggatccg attggtcatg ttgaagcaga aattattcag      540

gatctggtgg aaaaaggctt tattgttatt accagcggtg gtggcggtgt tccggttatt      600

gaagaggatg gtaaactgcg tggtgttgaa gccgttattg ataaagatct ggcaggcgaa      660

cgtctggcag aagaagttaa agcagatatc tttatgatcc tgaccgatgt taatggtgca      720

gccgttaatt ttggtaaacc ggatgaacgt tggctgggta aagttgcagt tgaagaactg      780

cgtaaatact atgaagaggg ccatttcaaa aaaggtagca tgggtccgaa agttctggca      840

gcaattcgtt ttgttgaatg gggtggtgaa cgtgcagtta ttgcagcact ggatcgtgcc      900

gttgaagcac tggaaggtaa aaccggcacc caggttatta aaggttaa                   948


<210>  17
<211>  948
<212>  DNA
<213>  Thermococcus sp.

<400>  17
atgaaacgtg ttgttattgc cctgggtggt aatgcaattc tgcagcgtgg tcagaaaggc       60

acctatgaag aacaaatggc caatgttatg aaaaccgcca aacaaatcgt ggatattatt      120

ctggatggcg attatgaagt ggtgattacc catggtaatg gtccgcaggt tggtgcactg      180

ctgctgcata tggatgcagg tcaggcaacc catggcattc cggcacagcc gatggatgtt      240

gccggtgcaa tgacccaggg tcagattggt tacatgattc agcaggcaat tcgcaatgaa      300

ctgaaacgtc gtggtattga tcgtccggtt gccaccattg ttacccagac cattgttgat      360

aaaaacgatc cggcatttca gcatccgagc aaaccggttg gtccgtttta tgatgaagaa      420

accgcaaaaa aactggccga agaaaaaggt tgggttgttg ttgaagatag cggtcgtggt      480

tggcgtcgtg ttgttccgag tccggatccg attggtcatg ttgaagcaga aattattcag      540

gatctggtgg aaaaaggctt tattgttatt accagcggtg gtggcggtgt tccggttatt      600

gaagaggatg gtaaactgcg tggtgttgaa gccgttattg ataaagatct ggcaggcgaa      660

cgtctggcag aagaagttaa agcagatatc tttatgatcc tgaccgatgt taatggtgca      720

gccgttaatt ttggtaaacc ggatgaacgt tggctgggta aagttgcagt tgaagaactg      780

cgtaaatact atgaagaggg ccatttcaaa aaaggtagca tgggtccgaa agttctggca      840

gcaattcgtt ttgttgaatg gggtggtgaa cgtgcagtta ttgcagcact ggatcgtgcc      900

gttgaagcac tggaaggtaa aaccggcacc caggttatta aaggttaa                   948


<210>  18
<211>  951
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Thermococcus 
       gammatolerans carbamate kinase

<400>  18
atgatcctga tgaaacgtgt tgttattgca ctgggtggta atgcaattct gcagcgtggt       60

cagcgtggca cctatgaaga acaaatggaa aatgttcgta aaaccgccaa acaaatcgcc      120

gatattattg aacgtggtta tgaagtggtt atcacccatg gtaatggtcc gcaggttggt      180

gcactgctgc tgcatatgga tgcaggtcag cagctgtatg gtattccggc acagccgatg      240

gatgttgccg gtgcaatgac ccagggtcag attggttaca tgattggtca ggcactgatt      300

aatgaactgc gtaaacgtgg tattgatcgt ccggttgcca ccattgttac ccagaccatt      360

gttgataaaa atgatccggc attcaaaaac ccgagcaaac cggttggtcc gttttatgat      420

gaagaaaccg caaaaaaact ggccaaagaa aaaggttggg tggttattga agatgccggt      480

cgtggttggc gtcgtgttgt tccgagtccg gatccgaaag gtcatgttga agcaccggtt      540

attcaggatc tggttgaaaa aggctttatt gtgattgcaa gcggtggtgg tggcgttccg      600

gtgattgaag aaaatggtga actgaaaggt gttgaagccg tgattgataa agatctggca      660

ggcgagaaac tggcagaaga agttaaagca gatatcttta tgattctgac cgatgttaat      720

ggcgcagcga ttaactttgg taaaccggat gaacgttggc tggaacgtgt taccgttgaa      780

gaactgcgca aatattacga agagggtcat tttaaacgcg gtagcatggg tccgaaagtt      840

ctggcagtta ttcgttttct ggaatggggt ggtgaacgtg caattattgc aagcctggat      900

cgtgcagttg aagccctgga aggtaaaacc ggcacccagg tttttccgta a               951


<210>  19
<211>  951
<212>  DNA
<213>  Thermococcus gammatolerans

<400>  19
atgatactaa tgaagagggt cgtcatagcc cttggcggta acgcaatcct ccagcgaggt       60

caaaggggaa cctacgagga gcagatggag aacgtgagaa agacggcgaa gcagatagct      120

gacataatcg agaggggcta cgaggtcgtt ataactcacg gaaacggtcc ccaggttggc      180

gcactactcc tccacatgga cgcgggccag cagctctacg gcataccggc ccagccgatg      240

gacgtggccg gtgcgatgac gcagggacag atcgggtaca tgatagggca ggccctaatc      300

aatgagctca gaaaacgcgg tatagacagg ccagttgcga cgatagtaac ccagacgatt      360

gtggacaaga atgaccccgc ctttaagaac ccgagcaagc ccgtcgggcc cttctacgac      420

gaggagacgg cgaagaagct ggccaaggag aagggctggg tggtcatcga ggatgcggga      480

aggggctgga ggcgtgttgt tccgagtccc gatcccaagg gccacgttga agccccggtg      540

atccaggatc tcgtggagaa gggcttcata gtcatcgcga gcggtggcgg tggcgttccc      600

gtcatagagg agaatggaga gcttaaaggc gtcgaggctg tcatagacaa ggatttggcg      660

ggggaaaagc tggccgagga ggtcaaggcg gatatcttca tgattctcac cgacgtcaac      720

ggtgccgcga taaacttcgg gaagccggac gagaggtggc ttgagagggt caccgtcgag      780

gagctcagga agtactacga agagggacac ttcaagaggg gcagcatggg tccgaaggtt      840

ctggccgtca taagattcct tgagtggggc ggtgaaaggg cgataatagc ctcgctcgac      900

agggccgttg aagccctcga agggaagacg ggaacgcagg tttttccctg a               951


<210>  20
<211>  948
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Thermococcus 
       kodakarensis carbamate kinase

<400>  20
atgaaacgtg ttgttattgc cctgggtggt aatgcaattc tgcagcgtgg tcagaaaggc       60

acctatgaag aacaaatgga aaatgttcgt cgtaccgcaa aacaaattgc cgatattatt      120

ctggatggcg attatgaagt tgtgattacc catggtaatg gtccgcaggt tggtgcactg      180

ctgctgcata tggatgcagg tcagcaggtt tatggtattc cggcacagcc gatggatgtt      240

gccggtgcaa tgacccaggg tcagattggt tacatgattg gtcaggcact gattaatgaa      300

ctgcgtaaac gtggtgttga aaaaccggtt gccaccattg ttacccagac cattgttgat      360

aaaaacgatc cggcatttca gaatccgagc aaaccggtgg gtccgtttta tgatgaagaa      420

accgcaaaaa aactggccaa agaaaaaggt tggaccgtta ttgaagatgc cggtcgtggt      480

tggcgtcgtg ttgttccgag tccggatccg aaaggtcatg ttgaagcacc ggttattgtt      540

gatctggttg aaaaaggctt tattgtgatt gcaagcggtg gtggtggcgt tccggtgatt      600

gaagaaaatg gtgaactgaa aggtgttgaa gccgtgattg ataaagatct ggcaggcgag      660

aaactggcag aagaagttaa agcagatatc ttcatgattc tgaccgatgt taatggtgca      720

gcgattaact atggtaaacc ggatgaaaaa tggctgggca aagttaccgt tgatgagctg      780

aaacgttatt acaaagaagg ccacttcaaa aaaggtagca tgggtccgaa agttctggca      840

gcaattcgtt ttgttgaatg gggtggtgaa cgtgcagtta ttgcaagcct ggatcgtgca      900

gttgaagccc tggaaggtaa aaccggcacc caggttgttc gtgaataa                   948


<210>  21
<211>  948
<212>  DNA
<213>  Thermococcus kodakarensis

<400>  21
atgaagagag ttgtcatagc cttgggcggg aacgccatac tccagcgagg ccagaagggg       60

acttacgagg agcagatgga gaacgtgagg aggactgcca agcagatcgc tgacataatc      120

cttgacggcg attatgaagt cgttatcacc cacggaaacg gtccacaggt tggtgcgctc      180

cttctccaca tggacgctgg ccagcaggtc tacggcatcc cagcccagcc tatggacgtc      240

gctggagcga tgacccaggg gcagataggc tacatgatcg gccaggcgct cataaacgag      300

cttaggaaga gaggggtcga gaagcccgtc gcaacgatag taacccagac catcgttgac      360

aagaacgacc cagccttcca gaacccgagc aagccggtcg gcccgttcta cgacgaggag      420

actgccaaga agctagcaaa ggaaaagggc tggacggtta tagaagacgc cgggaggggc      480

tggaggaggg tagttccgag tcccgacccg aaggggcacg tagaggctcc ggtcatagtt      540

gacctcgtcg agaagggctt catcgtcata gcgagcggcg gcggtggcgt cccagtgatt      600

gaggagaatg gagagcttaa gggcgttgag gcggttatag acaaagattt agccggtgaa      660

aagctggctg aagaggttaa agcggatatc ttcatgatac tcacggacgt gaacggcgcg      720

gcaataaact acggaaagcc cgatgagaag tggctcggga aggtaaccgt ggacgagctg      780

aagcgctatt acaaagaggg ccacttcaag aagggaagca tggggccgaa ggttcttgcc      840

gcgataaggt tcgttgagtg gggaggcgag agggcagtaa tagcttccct tgacagggcc      900

gtcgaggcgc ttgaggggaa gacggggacg caggttgtac gggagtga                   948


<210>  22
<211>  948
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Thermococcus 
       onnurineus carbamate kinase

<400>  22
atgaaacgtg ttgttattgc cctgggtggt aatgcaattc tgcagcgtgg tcagaaaggc       60

acctatgaag aacaaatgac caatgttatg aaaaccgcca aacaaatcgt ggatattatt      120

ctggatggcg attatgaagt ggtgattacc catggtaatg gtccgcagat tggtgcactg      180

ctgctgcata tggatgcagg tcagcagatt catggtattc cggcacagcc gatggatgtt      240

gccggtgcaa tgacccaggg tcagattggt tacatgattc agcaggcaat tcgcaatgaa      300

ctgaaacgtc gtggtgttga acgtccggtt gccaccattg ttacccagac cctggttgat      360

aaaaatgatc cggcatttca gaatccgagc aaaccggttg gtccgtttta tgatgaagaa      420

accgcaaaac gtctggccaa agaaaaaggt tggaccgtta ttgaagatag cggtcgtggt      480

tggcgtcgtg ttgttccgag tccggatccg attggtcatg ttgaaacacc ggttattcag      540

gatctggttg aaaaaggctt tattgtgatt gcaagcggtg gtggcggtgt tccggtgatt      600

gaagaggatg gtatgctgaa aggtgttgaa gcggttattg ataaagatct ggcaggcgaa      660

aaactggcag aagaagttaa cgcagatatc tttatgattc tgaccgatgt taatggcgca      720

gcgattaact atggtaaacc ggatgaaaaa tggctgggtc gtgttaccgt tgaagaactg      780

aaacgctatt acaatgaagg ccacttcaaa aaaggtagca tgggtccgaa agttctggca      840

gcaattcgtt ttgttgaatg gggtggtgaa cgtgcagtta ttgcagcact ggataaagca      900

gttgaagcac tggaaggtaa aaccggcacc caggttatta aaggttaa                   948


<210>  23
<211>  948
<212>  DNA
<213>  Thermococcus onnurineus

<400>  23
atgaagaggg tagttatagc tctcggcggg aacgcgattc ttcagcgagg tcaaaagggc       60

acttacgagg aacagatgac gaacgtcatg aagaccgcaa agcaaatcgt cgatataatc      120

ctcgatggcg attatgaggt tgtaatcacc catggaaacg gcccccagat tggtgccctt      180

ctcctccata tggatgctgg acagcagatt cacggtatcc cagcccagcc catggacgtt      240

gccggagcga tgactcaggg ccagatagga tacatgatcc agcaggcgat aagaaacgag      300

ctaaagagga ggggcgtgga gaggcccgtc gccaccatag tcacccagac gctcgttgac      360

aaaaacgacc cggctttcca gaacccgagc aagccagtcg gtccgttcta cgatgaggag      420

acggcaaaga ggcttgcaaa ggagaagggc tggacggtca tcgaagattc cggaaggggc      480

tggaggcgcg ttgtgccgag tccggacccg ataggtcacg tcgagacccc tgtgattcag      540

gatctagttg agaagggatt catagtcata gccagcggcg gcggtggcgt tcccgttatc      600

gaggaggatg gaatgctcaa gggagttgag gccgtcatag acaaagacct tgccggagag      660

aagctcgcag aagaggtaaa cgccgacatc ttcatgattc taaccgacgt gaacggtgcg      720

gcgataaact acggaaagcc cgacgagaag tggctcggaa gagttaccgt cgaagagctc      780

aagcgctact ataatgaggg ccacttcaag aagggcagca tggggccaaa ggttctcgcc      840

gctatacgct tcgtcgagtg gggcggcgag agggcggtta tagccgcgct ggataaggcc      900

gtggaagcgc ttgaaggcaa aaccgggact caggtcataa agggctga                   948


<210>  24
<211>  948
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Thermococcus 
       barophilus carbamate kinase

<400>  24
atgcgtaaac gtgttgttat tgcactgggt ggtaatgcaa ttctgcagcg tggtcagaaa       60

ggcacctatg atgaacaaat ggaaaatgtg aaaaaaaccg ccaaacaaat tgtggatatt      120

attctgaata atgattatga agtggtgatt acccatggta atggtccgca ggttggtgca      180

ctgctgctgc acatggatgc aggtcagcag ctgtatggta ttccggcaca gccgatggat      240

gttgccggtg caatgaccca gggtcagatt ggttacatga ttcagcaggc aattaccaat      300

gaactgaaac gtcgcggtat ctataaaccg gttgcaacca ttgttaccca ggttctggtt      360

gataaaaatg atccggcatt tcagaatccg agcaaaccgg ttggtccgtt ttatgatgaa      420

gaaaccgcaa aacgtctggc caaagaaaaa gaatgggttg ttgttgaaga tgcaggtcgt      480

ggttggcgtc gtgttgttcc gagtccggat ccgaaagata ttattgaaaa agatattatt      540

cgcgatctgg tggaaaaagg ctttattgtt attgcaagcg gtggtggtgg tattccggtt      600

attgaagaaa atggtcagct gaaaggtgtt gaagccgtga ttgataaaga tctggcaggc      660

gaaaaactgg ccgaagttgt taatgcagat atctttatga ttctgaccga tgtgaatggc      720

gcagccatta actatggtaa accgaatgaa cgttggctgc ataaagttgc agttgatgaa      780

ctgcgcaaat actatgaaga aggccatttc aaaaaaggca gcatgggtcc gaaagttctg      840

gcagcaattc gttttgttga atggggtggt gaacgtgcag ttattgcagc actggataaa      900

gcagttgatg cactggaagg tcgtaccggc acccaggtga ttaaaatg                   948


<210>  25
<211>  951
<212>  DNA
<213>  Thermococcus barophilus

<400>  25
atgaggaaga gggttgttat tgccttgggc ggaaacgcta ttcttcagcg tggtcagaag       60

gggacttatg atgagcagat ggaaaatgta aaaaaaactg caaagcaaat tgtcgatata      120

atccttaaca acgattatga ggttgtaatt actcatggaa acggtcctca ggttggagcc      180

ttgcttctcc acatggatgc tgggcaacaa ctttatggga ttccagctca gccaatggat      240

gttgctggag ctatgactca gggtcagata ggctacatga tacagcaggc tataacaaac      300

gagcttaaac ggagggggat ttacaagcct gttgcaacaa ttgtgacaca ggttcttgtt      360

gacaagaacg atccagcttt tcagaatccg tcaaagccag ttggaccatt ctatgatgag      420

gaaacggcaa aaaggctcgc caaagagaaa gagtgggttg ttgtggaaga cgctggtaga      480

ggatggcgta gagttgttcc atctccggat ccaaaggata taattgagaa ggacataatc      540

agggatttag ttgagaaagg cttcattgtc atagcgtcgg gtggtggggg aattccggtt      600

atagaggaaa acggacagct caaaggtgtt gaggctgtca ttgataagga tctggctgga      660

gaaaagctgg ctgaggttgt taatgctgac atcttcatga ttctcactga tgtaaatggg      720

gctgcaataa attatggaaa gccgaatgaa aggtggctcc acaaagttgc tgttgatgag      780

ctcaggaagt attatgaaga ggggcacttt aagaaaggca gcatggggcc taaggtctta      840

gcagcgataa gatttgtgga atggggcggg gagagagcgg tcattgctgc tcttgataaa      900

gcagttgatg cattggaagg cagaactgga acccaagtaa tcaaaatgtg a               951


<210>  26
<211>  945
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Thermococcus 
       sibiricus carbamate kinase

<400>  26
atgcgtaaac gtgttgttat tgcactgggt ggtaatgcaa ttctgcagcg tggtcagaaa       60

ggcacctatg aagaacaaat ggaaaatgtt cgtaaaaccg cacgtcagat tgtggatatt      120

attctggata atgaatatga agtggtgatt acccatggta atggtccgca ggttggtgca      180

ctgctgcttc agcaggatgc cggtgaacat gtgcatggta ttccggcaca gccgatggat      240

gtttgtggtg caatgagcca gggtcagatt ggttatatga ttcagcaggc cattatgaat      300

gaactgcgtc gtcgtggtgt tgaacgtccg gttgcaacca ttgttaccca gaccattgtt      360

gataaaaatg atccggcatt tcagcatccg agcaaaccgg ttggtccgtt ttatagcgaa      420

gaaaccgcaa aaaaactggc caaagaaaaa ggttgggtgg ttattgaaga tgcaggtcgt      480

ggttggcgtc gtgttgttcc gagtccggat ccgaaaggtc atgttgaagc accgattatt      540

caggatctgg tggaaaaaga atttattgtg attagcagcg gtggtggtgg tattccggtt      600

gttgaagaaa atggtgaact gaaaggtgtt gaagccgtga ttgataaaga tctggcaggc      660

gaacgtctgg ccgaagaagt taacgcagat atctttatga ttctgaccga tgtgaatggc      720

gcagccatta actatggtcg tccgaatgaa aaatggctgg aaaaagttac cctgggcgaa      780

attaaacgct actatgaaga aggccatttc aaaaaaggta gcatgggtcc gaaagttctg      840

gcagcaattc gttttattga atggggtggt gaacgtgcaa ttattgcagc actggataaa      900

gcagttgaag cactggaagg taaaaccggc acccagatta cccgt                      945


<210>  27
<211>  948
<212>  DNA
<213>  Thermococcus sibiricus

<400>  27
atgagaaaga gagttgtcat agctttaggt gggaatgcta ttcttcaaag gggtcagaaa       60

ggtacttatg aagagcaaat ggagaatgtg agaaagactg caaggcagat tgttgatatt      120

attcttgata atgagtatga agtggttatc actcatggta atggtcctca agttggagct      180

cttcttctcc aacaggatgc tggagagcac gtgcatggaa ttcctgctca acctatggat      240

gtttgtggtg caatgagtca gggtcaaata ggttacatga ttcagcaggc aataatgaat      300

gaattgagga ggaggggtgt tgaacggcca gtggcgacta tagtcaccca aactattgtg      360

gacaagaatg atcccgcttt tcagcaccca tcaaaaccag ttgggccctt ctatagtgaa      420

gagactgcta aaaaactcgc taaggagaaa ggttgggtgg ttatagagga cgctggaagg      480

ggttggagga gggtagtgcc aagtccagac ccaaagggac atgtagaggc tcccataatc      540

caagatcttg ttgaaaaaga atttatagtg atatcttcag gtggtggagg aattcctgtt      600

gtagaggaga atggtgagct taagggcgtt gaagcagtta ttgacaaaga tctggctgga      660

gaaaggcttg ctgaggaagt taacgctgat attttcatga ttcttacgga tgttaatgga      720

gctgcaatta actatggtag acctaacgaa aagtggcttg aaaaggttac tttgggagaa      780

ataaagaggt attatgagga aggccacttc aaaaagggta gcatggggcc aaaagtactt      840

gcagcaataa ggtttattga gtggggtggg gagagggcaa taatagcggc actggataag      900

gctgttgagg cattggaagg aaaaacaggc acccaaataa caagatga                   948


<210>  28
<211>  311
<212>  PRT
<213>  Fervidobacterium nodosum

<400>  28

Met Lys Lys Leu Ala Val Val Ala Ile Gly Gly Asn Ala Val Asn Arg 
1               5                   10                  15      


Pro Gly Glu Glu Pro Thr Ala Glu Asn Met Ile Lys Asn Leu Ser Glu 
            20                  25                  30          


Thr Ala His Tyr Leu Ala Gly Met Leu Asp Glu Tyr Asp Ile Ile Ile 
        35                  40                  45              


Thr His Gly Asn Gly Pro Gln Val Gly Asn Leu Leu Val Gln Gln Asp 
    50                  55                  60                  


Leu Ala Lys His Val Ile Pro Pro Phe Pro Ile Asp Val Asn Asp Ala 
65                  70                  75                  80  


Gln Thr Gln Gly Ser Leu Gly Tyr Met Ile Ala Leu Thr Leu Glu Asn 
                85                  90                  95      


Glu Leu Lys Arg Arg Asn Ile Glu Arg Gln Ile Ala Ala Ile Val Thr 
            100                 105                 110         


Gln Ile Glu Val Asp Lys Asn Asp Pro Ala Phe Gln Lys Pro Thr Lys 
        115                 120                 125             


Pro Val Gly Pro Phe Tyr Ser Lys Glu Glu Ala Glu Lys Leu Ala Gln 
    130                 135                 140                 


Glu Lys Gly Trp Ile Met Lys Glu Asp Ala Gly Arg Gly Tyr Arg Arg 
145                 150                 155                 160 


Val Val Pro Ser Pro Ile Pro Leu Asp Ile Val Glu Lys Glu Val Ile 
                165                 170                 175     


Lys Met Leu Val Glu Lys Asp Val Ile Val Ile Ala Ala Gly Gly Gly 
            180                 185                 190         


Gly Ile Pro Val Val Lys Glu Asn Gly Met Phe Lys Gly Val Glu Ala 
        195                 200                 205             


Val Ile Asp Lys Asp Arg Ala Ser Ala Leu Leu Ala Lys Glu Val Glu 
    210                 215                 220                 


Ala Asp Ile Leu Ile Ile Leu Thr Gly Val Glu Lys Val Cys Ile Asn 
225                 230                 235                 240 


Tyr Lys Lys Pro Asp Gln Phe Glu Val Asp Lys Leu Thr Val Glu Glu 
                245                 250                 255     


Ala Lys Lys Tyr Leu Ala Glu Gly Gln Phe Pro Ser Gly Ser Met Gly 
            260                 265                 270         


Pro Lys Ile Glu Ala Ala Ile Asp Phe Val Ser Ser Thr Gly Arg Glu 
        275                 280                 285             


Cys Ile Ile Thr Asp Met Ala Val Leu Asp Lys Ala Leu Lys Gly Glu 
    290                 295                 300                 


Thr Gly Thr Lys Ile Val Pro 
305                 310     


<210>  29
<211>  312
<212>  PRT
<213>  Thermosipho melanesiensis

<400>  29

Met Lys Lys Leu Ala Val Val Ala Ile Gly Gly Asn Ala Val Asn Arg 
1               5                   10                  15      


Pro Gly Glu Lys Pro Thr Ala Glu Asn Met Leu Lys Asn Leu Ser Glu 
            20                  25                  30          


Thr Ala Lys Tyr Ile Val Asn Met Ile Asp Glu Tyr Asp Val Ala Ile 
        35                  40                  45              


Thr His Gly Asn Gly Pro Gln Val Gly Asn Leu Leu Val Gln Gln Glu 
    50                  55                  60                  


Ile Ala Lys Asp Lys Ile Pro Pro Phe Pro Ile Asp Val Asn Asp Ala 
65                  70                  75                  80  



Gln Thr Gln Gly Ser Leu Gly Tyr Met Ile Ala Gln Ser Ile Arg Asn 
                85                  90                  95      


Gln Leu Lys Ala Ile Gly Lys Asp Met Glu Ile Ser Ala Val Val Thr 
            100                 105                 110         


Gln Ile Ile Val Asp Lys Asn Asp Pro Ala Phe Gln Asn Pro Thr Lys 
        115                 120                 125             


Pro Val Gly Pro Phe Tyr Thr Glu Glu Glu Ala Lys Met Leu Glu Lys 
    130                 135                 140                 


Glu Lys Gly Trp Lys Ile Val Glu Asp Ala Gly Arg Gly Trp Arg Arg 
145                 150                 155                 160 


Val Val Pro Ser Pro Lys Pro Leu Asp Ile Val Glu Lys Asp Val Ile 
                165                 170                 175     


Lys Leu Leu Met Lys Asn Asp Val Met Val Ile Ala Ala Gly Gly Gly 
            180                 185                 190         


Gly Ile Pro Val Ile Val Glu Asp Asn Lys Leu Lys Gly Val Glu Ala 
        195                 200                 205             


Val Ile Asp Lys Asp Arg Ala Ser Ala Leu Leu Ala Lys Glu Val Asp 
    210                 215                 220                 


Ala Asp Ile Leu Ile Ile Leu Thr Gly Val Glu Lys Val Tyr Leu Asn 
225                 230                 235                 240 


Phe Gly Lys Glu Asp Gln Lys Ala Leu Asp Lys Ile Thr Thr Thr Glu 
                245                 250                 255     


Ala Lys Lys Phe Leu Gln Glu Gly His Phe Pro Lys Gly Ser Met Gly 
            260                 265                 270         


Pro Lys Ile Glu Ala Ala Ile Asp Phe Val Glu Ser Thr Gly Lys Glu 
        275                 280                 285             


Cys Leu Ile Thr Asp Met Ser Val Leu Asp Lys Ala Leu Arg Gly Glu 
    290                 295                 300                 


Thr Gly Thr Arg Ile Ile Lys Gly 
305                 310         


<210>  30
<211>  323
<212>  PRT
<213>  Anaerobaculum hydrogeniformans

<400>  30

Met Met Ala Glu Lys Val Val Val Ala Leu Gly Gly Asn Ala Ile Leu 
1               5                   10                  15      


Gln Ser Gly Gln Lys Gly Thr Phe Glu Glu Gln Met Glu Asn Val Met 
            20                  25                  30          


Ala Thr Ala Arg Gln Ile Val Arg Met Leu Glu Ala Gly Tyr Glu Val 
        35                  40                  45              



Val Val Thr His Gly Asn Gly Pro Gln Val Gly Ala Ile Leu Ile Gln 
    50                  55                  60                  


Asn Glu Leu Gly Ser Gly Leu Val Pro Ser Met Pro Met Asp Val Cys 
65                  70                  75                  80  


Gly Ala Glu Ser Gln Gly Met Ile Gly Tyr Met Leu Cys Gln Ala Leu 
                85                  90                  95      


Arg Asn Cys Met Leu Gly Lys Ser Leu Asn Asp Trp Gln Pro Cys Cys 
            100                 105                 110         


Met Val Thr Gln Val Glu Val Asp Pro Asn Asp Pro Ala Phe Ala Lys 
        115                 120                 125             


Pro Thr Lys Pro Val Gly Pro Phe Tyr Thr Ala Glu Glu Ala Lys Lys 
    130                 135                 140                 


Arg Met Ser Gly Lys Asn Glu Ile Trp Ile Glu Asp Ser Gly Arg Gly 
145                 150                 155                 160 


Trp Arg Arg Val Val Pro Ser Pro Asp Pro Lys Lys Ile Val Glu Gly 
                165                 170                 175     


Glu Val Ile Lys His Leu Ser Asp Glu Arg Tyr Leu Val Ile Ala Cys 
            180                 185                 190         


Gly Gly Gly Gly Ile Pro Val Ile Lys Asp Glu Asp Gly Thr Tyr Arg 
        195                 200                 205             


Gly Val Glu Ala Val Ile Asp Lys Asp Leu Ala Gly Glu Arg Leu Ala 
    210                 215                 220                 


Gln Glu Val Gly Ala Asp Ile Phe Met Ile Leu Thr Asp Val Pro Lys 
225                 230                 235                 240 


Val Ala Ile Asn Tyr Lys Lys Pro Asp Glu Lys Trp Leu Asp Val Val 
                245                 250                 255     


Thr Pro Glu Glu Leu Arg Ala Tyr Glu Ala Glu Gly His Phe Lys Ala 
            260                 265                 270         


Gly Ser Met Gly Pro Lys Val Lys Ala Ala Leu Arg Phe Val Glu Asn 
        275                 280                 285             


Gly Gly Lys Arg Ala Ile Ile Ala Lys Leu Asp Ser Ala Leu Glu Ala 
    290                 295                 300                 


Leu Glu Gly Lys Thr Gly Thr Gln Val Val Pro Val Lys Glu Lys Ile 
305                 310                 315                 320 


Cys Cys Arg 
            


<210>  31
<211>  315
<212>  PRT
<213>  Aminobacterium colombiense

<400>  31

Met Gly Lys Arg Ile Leu Val Ala Leu Gly Gly Asn Ala Ile Leu Gln 
1               5                   10                  15      


Pro Gly Gln Lys Gly Thr Ser Ala Glu Gln Val Thr Asn Ile Asp Lys 
            20                  25                  30          


Thr Val Lys Gln Leu Ile Asp Val Val Glu Lys Gly Tyr Glu Leu Val 
        35                  40                  45              


Leu Thr His Gly Asn Gly Pro Gln Val Gly Ala Ile Leu Ile Gln His 
    50                  55                  60                  


Glu Met Ala Lys Glu Ala Ile Pro Ala Met Pro Leu Asp Phe Cys Gly 
65                  70                  75                  80  


Ala Glu Ser Gln Gly Leu Ile Gly Tyr Met Leu Cys Gln Ser Ile Arg 
                85                  90                  95      


Asn Glu Cys Ala Gly Arg Gly Leu Lys Lys Glu Ala Val Cys Ile Val 
            100                 105                 110         


Thr Gln Val Glu Val Asp Pro His Asp Lys Ala Phe Arg Asn Pro Thr 
        115                 120                 125             


Lys Pro Val Gly Pro Phe Tyr Thr Gln Ala Glu Ala Glu Gln Asn Met 
    130                 135                 140                 


Ala Glu Arg Gly Glu Arg Trp Ile Glu Asp Ala Gly Arg Gly Trp Arg 
145                 150                 155                 160 


Lys Val Val Pro Ser Pro Glu Pro Lys Glu Ile Val Glu Lys Glu Val 
                165                 170                 175     


Ile Arg Ala Leu Val Asp Gln Gly Thr Val Val Ile Ala Ser Gly Gly 
            180                 185                 190         


Gly Gly Ile Pro Val Cys Arg Asn Asp Gln Gly Gln Leu Tyr Gly Val 
        195                 200                 205             


Glu Ala Val Ile Asp Lys Asp Leu Ala Gly Glu Arg Leu Ala Leu Asp 
    210                 215                 220                 


Val Gly Ala Asp Ile Phe Val Ile Leu Thr Asp Val Ser His Ala Ile 
225                 230                 235                 240 


Leu His Tyr Asn Thr Pro Gln Gln Lys Pro Leu Lys Lys Val Thr Leu 
                245                 250                 255     


Glu Glu Met Lys Lys Tyr Ile Glu Glu Gly His Phe Arg Ala Gly Ser 
            260                 265                 270         


Met Gly Pro Lys Val Arg Ala Ala Leu Asn Phe Val Lys Lys Gly Gly 
        275                 280                 285             


Gly Arg Ala Ile Ile Ala Arg Leu Asp Gln Val Ile Pro Ala Leu Lys 
    290                 295                 300                 


Gly Glu Val Gly Thr Gln Ile Glu Ser Thr Leu 
305                 310                 315 


<210>  32
<211>  318
<212>  PRT
<213>  Thermanaerovibrio acidaminovorans

<400>  32

Met Ala Ala Thr Lys Val Val Val Ala Leu Gly Gly Asn Ala Leu Gln 
1               5                   10                  15      


Glu Ala Gly Thr Pro Pro Thr Ala Glu Ala Gln Leu Glu Val Val Lys 
            20                  25                  30          


Lys Thr Ala Thr Tyr Leu Ala Glu Ile Ser Lys Arg Gly Tyr Glu Met 
        35                  40                  45              


Ala Val Val His Gly Asn Gly Pro Gln Val Gly Arg Ile Val Leu Ser 
    50                  55                  60                  


Gln Glu Ile Ala Ala Gln Ala Asn Pro Glu Thr Pro Ala Met Pro Phe 
65                  70                  75                  80  


Asp Val Cys Gly Ala Met Ser Gln Gly Tyr Ile Gly Tyr Gln Ile Gln 
                85                  90                  95      


Gln Ala Leu Arg Asp Ala Leu Arg Asn Arg Asn Leu Asn Ile Pro Val 
            100                 105                 110         


Val Thr Leu Val Thr Gln Val Val Val Asp Ala Asn Asp Pro Ala Phe 
        115                 120                 125             


Lys Asn Pro Thr Lys Pro Ile Gly Pro Phe Tyr Thr Glu Glu Glu Ala 
    130                 135                 140                 


Lys Lys Leu Met Ala Glu Lys Gly Tyr Val Met Lys Glu Asp Ala Gly 
145                 150                 155                 160 


Arg Gly Trp Arg Arg Val Val Ala Ser Pro Glu Pro Lys Lys Ile Thr 
                165                 170                 175     


Glu Ile Ser Ala Val Lys Arg Leu Trp Asp Thr Thr Ile Val Val Thr 
            180                 185                 190         


Ala Gly Gly Gly Gly Ile Pro Val Val Glu Asn Met Asp Gly Ser Leu 
        195                 200                 205             


Ser Gly Val Ala Ala Val Ile Asp Lys Asp Leu Ala Ala Glu Lys Leu 
    210                 215                 220                 


Ala Glu Glu Ile Glu Ala Asp Ile Leu Leu Ile Leu Thr Glu Val Asp 
225                 230                 235                 240 


Lys Val Cys Ile Asn Phe Lys Lys Pro Asn Gln Gln Glu Leu Ser His 
                245                 250                 255     


Met Thr Val Ala Glu Ala Ile Lys Tyr Met Glu Glu Gly His Phe Ala 
            260                 265                 270         


Pro Gly Ser Met Leu Pro Lys Val Met Ala Ala Val Lys Phe Ala Arg 
        275                 280                 285             


Thr Phe Pro Gly Lys Lys Ala Ile Ile Thr Ser Leu Tyr Lys Ala Val 
    290                 295                 300                 


Asp Ala Leu Glu Gly Arg Glu Gly Thr Val Val Thr Met Ala 
305                 310                 315             


<210>  33
<211>  310
<212>  PRT
<213>  Halothermothrix orenii

<400>  33

Met Ala Arg Ile Val Ile Ala Leu Gly Gly Asn Ala Leu Gly Lys Thr 
1               5                   10                  15      


Pro Leu Glu Gln Arg Glu Ala Val Lys Asn Thr Ala Gln Pro Ile Val 
            20                  25                  30          


Asp Leu Ile Glu Asp Gly His Glu Ile Ile Leu Ala His Gly Asn Gly 
        35                  40                  45              


Pro Gln Val Gly Met Ile Asn Leu Ala Phe Glu Thr Ala Ala Gln Asn 
    50                  55                  60                  


Asp Asp Asn Val Pro Glu Met Pro Phe Pro Glu Cys Gly Ala Met Ser 
65                  70                  75                  80  


Gln Gly Tyr Ile Gly Tyr His Leu Gln Asn Ala Ile Arg Asn Glu Leu 
                85                  90                  95      


Val Asn Arg Gly Ile Asn Lys Ser Ile Thr Ser Val Val Thr Gln Val 
            100                 105                 110         


Leu Val Asp Lys Asn Asp Asn Ala Phe Glu His Pro Thr Lys Pro Val 
        115                 120                 125             


Gly Ser Phe Tyr Thr Glu Asp Glu Ala Arg Lys Leu Ile Glu Glu Lys 
    130                 135                 140                 


Gly Tyr Arg Met Val Glu Asp Ser Gly Arg Gly Tyr Arg Arg Val Val 
145                 150                 155                 160 


Pro Ser Pro Ile Pro Val Asp Ile Val Glu Lys Glu Ile Ile Lys Asn 
                165                 170                 175     


Leu Val Glu Asp Gly Asn Ile Val Ile Ala Cys Gly Gly Gly Gly Val 
            180                 185                 190         


Pro Val Val Glu Asp Glu Glu Gly Leu Lys Gly Val Pro Ala Val Ile 
        195                 200                 205             


Asp Lys Asp Phe Ala Ser Glu Lys Met Ala Glu Ile Val Asn Ala Asp 
    210                 215                 220                 


Leu Leu Val Ile Leu Thr Ala Val Glu Lys Val Ala Ile Asn Phe Gly 
225                 230                 235                 240 


Lys Glu Asn Glu Glu Trp Leu Ser Glu Met Asn Val Glu Leu Ala Gln 
                245                 250                 255     


Gln Tyr Cys Asp Glu Gly His Phe Ala Pro Gly Ser Met Leu Pro Lys 
            260                 265                 270         


Val Lys Ala Ala Met Lys Phe Ala Ser Ser Lys Glu Gly Arg Lys Ala 
        275                 280                 285             


Leu Ile Thr Ser Leu Glu Lys Ala Lys Glu Gly Leu Ala Gly Lys Thr 
    290                 295                 300                 


Gly Thr Leu Val Thr Ala 
305                 310 


<210>  34
<211>  312
<212>  PRT
<213>  Kosmotoga olearia

<400>  34

Met Lys Lys Ala Val Val Ala Ile Gly Gly Asn Ala Leu Asn Lys Pro 
1               5                   10                  15      


Gly Glu Lys Pro Ser Ala Glu Ala Met Lys Lys Asn Leu Leu Gly Thr 
            20                  25                  30          


Val Lys His Leu Ala Asp Leu Ile Glu Asp Gly Tyr Asp Leu Val Ile 
        35                  40                  45              


Thr His Gly Asn Gly Pro Gln Val Gly Asn Leu Leu Val Gln Gln Glu 
    50                  55                  60                  


Ile Ala Lys Asp Thr Leu Pro Pro Phe Pro Ile Asp Val Asn Asp Ala 
65                  70                  75                  80  


Met Thr Gln Gly Ser Ile Gly Tyr Leu Ile Thr Gln Thr Leu Gly Asn 
                85                  90                  95      


Glu Leu Lys Ser Arg Gly Thr Glu Arg Gln Ile Ala Cys Val Leu Thr 
            100                 105                 110         


Gln Ile Ile Val Asp Arg Asn Asp Pro Gly Phe Glu Asn Pro Thr Lys 
        115                 120                 125             


Pro Val Gly Pro Phe Tyr Asp Glu Glu Thr Ala Lys Lys Leu Gln Ala 
    130                 135                 140                 


Glu Lys Gly Trp Ile Met Lys Glu Asp Ala Gly Arg Gly Trp Arg Arg 
145                 150                 155                 160 


Val Val Pro Ser Pro Lys Pro Leu Asp Val Val Glu Ile Glu Ala Ile 
                165                 170                 175     


Lys Ala Leu Thr Gly Asn Asp Phe Ile Leu Val Ala Gly Gly Gly Gly 
            180                 185                 190         


Gly Ile Pro Val Val Lys Lys Asp Asp Gly Thr Leu Glu Gly Val Glu 
        195                 200                 205             


Ala Val Ile Asp Lys Asp Arg Ala Ser Ala Leu Leu Ala Arg Leu Leu 
    210                 215                 220                 


Asp Ala Asp Leu Phe Leu Ile Leu Thr Ala Val Asp His Ala Tyr Ile 
225                 230                 235                 240 


Asn Phe Gly Lys Pro Asp Gln Lys Ala Leu Glu Arg Ile Ser Val Asn 
                245                 250                 255     


Glu Ala Lys Lys Leu Met Asp Glu Gly His Phe Ala Lys Gly Ser Met 
            260                 265                 270         


Tyr Pro Lys Ile Glu Ser Ala Ile Asp Phe Val Glu Ser Thr Gly Lys 
        275                 280                 285             


Glu Ala Ile Ile Thr Ser Leu Glu Lys Val Lys Glu Ala Ile Gln Gly 
    290                 295                 300                 


Lys Ser Gly Thr His Ile Thr Lys 
305                 310         


<210>  35
<211>  312
<212>  PRT
<213>  Moorella thermoacetica

<400>  35

Met Glu Arg Leu Ala Val Ile Ala Ile Gly Gly Asn Ser Leu Ile Lys 
1               5                   10                  15      


Asn Lys Lys Leu Ile Ser Leu Tyr Asp Gln Leu Ala Thr Met Arg Glu 
            20                  25                  30          


Thr Cys Arg Asn Ile Ala Asp Met Val Glu Lys Gly Trp Asn Val Val 
        35                  40                  45              


Ile Thr His Gly Asn Gly Pro Gln Val Gly Phe Leu Ile Arg Arg Ala 
    50                  55                  60                  


Glu Leu Ala Cys Gly Glu Leu Pro Leu Ile Pro Leu Glu Phe Ala Val 
65                  70                  75                  80  


Ala Asp Thr Gln Gly Ala Ile Gly Tyr Met Ile Gln Gln Ser Leu Met 
                85                  90                  95      


Asn Glu Phe Arg Arg Arg Gly Ile Lys Arg Gln Ala Ile Thr Ile Val 
            100                 105                 110         


Thr Gln Val Val Val Asp Gln Asn Asp Glu Ala Phe Gln Asn Pro Thr 
        115                 120                 125             


Lys Pro Ile Gly Ser Phe Met Thr Arg Glu Glu Ala Gln Arg His Val 
    130                 135                 140                 


Glu Glu Asp Gly Trp Val Val Val Glu Asp Ala Gly Arg Gly Trp Arg 
145                 150                 155                 160 


Arg Val Val Pro Ser Pro Glu Pro Lys Ala Ile Val Glu Ile Arg Ala 
                165                 170                 175     


Ile Lys Asp Leu Ile Glu Asp Gly Tyr Ile Val Ile Cys Thr Gly Gly 
            180                 185                 190         


Gly Gly Ile Pro Val Val Glu His Asp Gly Asn Leu Lys Gly Val Ala 
        195                 200                 205             


Ala Val Val Asp Lys Asp Asn Ala Ser Ala Leu Leu Ala Asn Glu Ile 
    210                 215                 220                 


Lys Ala Asp Val Leu Val Ile Ser Thr Gly Val Asn Lys Val Ala Ile 
225                 230                 235                 240 


Asn Phe Gly Arg Pro Asp Gln Lys Glu Leu Asp Arg Leu Thr Ile Ala 
                245                 250                 255     


Glu Ala Glu Ala Tyr Met Ala Ala Gly His Phe Pro Pro Gly Asn Met 
            260                 265                 270         


Gly Pro Lys Ile Thr Ala Leu Leu Arg Tyr Leu Lys Asn Gly Gly Lys 
        275                 280                 285             


Glu Gly Ile Ile Thr Ser Pro Thr Glu Leu Val Ala Ala Leu Glu Gly 
    290                 295                 300                 


Lys Ser Gly Thr Arg Ile Val Leu 
305                 310         


<210>  36
<211>  933
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Fervidobacterium 
       nodosum carbamate kinase

<400>  36
atgaaaaaac tggccgttgt tgccattggt ggtaatgcag ttaatcgtcc gggtgaagaa       60

ccgaccgcag aaaatatgat taaaaacctg agcgaaaccg cacattatct ggcaggtatg      120

ctggatgaat atgatattat cattacccat ggcaatggtc cgcaggttgg taatctgctg      180

gttcagcagg atctggcaaa acatgttatt cctccatttc cgattgatgt taatgatgca      240

cagacccagg gtagcttagg ttatatgatt gcactgaccc tggaaaatga actgaaacgt      300

cgtaatattg aacgtcagat tgcagcaatt gtgacccaga ttgaagtgga taaaaatgat      360

ccggcatttc agaaaccgac caaaccggtg ggtccgtttt atagcaaaga agaagcagaa      420

aaactggccc aagaaaaagg ttggattatg aaagaagatg caggtcgcgg ttatcgtcgt      480

gttgttccga gcccgattcc gctggatatt gttgaaaaag aagtgattaa aatgctggtt      540

gaaaaagatg ttattgtgat tgcagccggt ggtggtggta ttccggttgt taaagaaaat      600

ggtatgttta aaggtgtgga agccgtgatt gataaagatc gtgcaagcgc actgctggca      660

aaagaagttg aagcagatat tctgattatt ctgaccggtg ttgaaaaagt gtgcattaat      720

tacaaaaaac cggatcagtt tgaagttgat aaactgaccg ttgaagaagc caaaaaatac      780

ctggcagaag gtcagtttcc gagcggtagc atgggtccga aaattgaagc agcaattgat      840

tttgttagca gcaccggtcg tgaatgcatt attaccgata tggcagttct ggataaagcc      900

ctgaaaggtg aaaccggcac caaaattgtt ccg                                   933


<210>  37
<211>  936
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Thermosipho 
       melanesiensis carbamate kinase

<400>  37
atgaaaaaac tggccgttgt tgccattggt ggtaatgcag ttaatcgtcc gggtgaaaaa       60

ccgaccgcag aaaatatgct gaaaaatctg agcgaaaccg ccaaatatat cgtgaatatg      120

attgatgaat atgatgtggc cattacccat ggcaatggtc cgcaggttgg taatctgctg      180

gttcagcaag aaattgccaa agataaaatt cctccgtttc cgattgatgt taatgatgca      240

cagacccagg gtagtctggg ttatatgatt gcacagagca ttcgtaatca gctgaaagcc      300

attggtaaag atatggaaat tagcgcagtt gtgacccaga ttattgtgga taaaaatgat      360

ccggcatttc agaatccgac caaacctgtt ggtccgtttt ataccgaaga agaggcaaaa      420

atgctggaaa aagaaaaagg ctggaaaatt gttgaagatg caggtcgtgg ttggcgtcgt      480

gttgttccga gcccgaaacc gctggatatt gttgaaaaag atgtgattaa actgctgatg      540

aaaaatgatg ttatggtgat tgcagccggt ggtggtggta ttccggttat tgtggaagat      600

aacaaactga aaggtgtgga agccgtgatt gataaagatc gtgcaagcgc actgctggca      660

aaagaagttg acgcagatat tctgattatt ctgaccggtg tggaaaaagt gtatctgaat      720

tttggcaaag aagatcagaa agccctggat aaaatcacca ccaccgaagc caaaaaattc      780

ttacaagaag gtcattttcc gaaaggtagc atgggtccga aaattgaagc agccattgat      840

tttgttgaaa gcaccggtaa agaatgcctg attaccgata tgagcgttct ggataaagca      900

ctgcgtggtg aaaccggcac ccgtattatc aaaggt                                936


<210>  38
<211>  972
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Anaerobaculum 
       hydrogeniformans carbamate kinase

<400>  38
atgatggccg aaaaagttgt tgttgcactg ggtggtaatg caattctgca gagcggtcag       60

aaaggcacct ttgaagaaca aatggaaaat gttatggcaa ccgcacgtca gattgttcgt      120

atgctggaag caggttatga agttgtggtt acccatggta atggtccgca ggttggtgcc      180

attctgattc agaacgaact gggtagcggt ctggttccga gcatgccgat ggatgtttgt      240

ggtgcagaaa gccagggtat gattggttat atgctgtgtc aggcactgcg taattgtatg      300

ctgggtaaaa gcctgaatga ttggcagccg tgttgtatgg tgacccaggt tgaagttgat      360

ccgaatgatc cggcatttgc aaaaccgacc aaaccggtgg gtccgtttta taccgcagaa      420

gaggcaaaaa aacgtatgag cggcaaaaac gaaatctgga ttgaagatag tggtcgtggt      480

tggcgtcgtg ttgttccgag tccggatccg aaaaaaatcg ttgaaggtga agtgatcaaa      540

cacctgagtg atgaacgtta tctggttatt gcatgcggtg gtggtggtat tccggttatt      600

aaagatgaag atggcaccta tcgtggtgtt gaagcagtta ttgataaaga tctggcaggc      660

gaacgtctgg cacaagaagt tggcgcagat atctttatga ttctgaccga tgttccgaaa      720

gtggccatca attacaaaaa accggatgag aaatggctgg atgttgttac accggaagaa      780

ctgcgtgcat atgaagcaga aggtcatttt aaagcaggta gcatgggtcc gaaagttaaa      840

gcagcactgc gttttgttga aaatggtggt aaacgtgcca ttatcgcaaa actggatagc      900

gcactggaag ccctggaagg taaaaccggt acacaggttg ttccggttaa agaaaaaatc      960

tgctgtcgct aa                                                          972


<210>  39
<211>  948
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Aminobacterium 
       colombiense carbamate kinase

<400>  39
atgggtaaac gtattctggt tgcactgggt ggtaatgcaa ttctgcagcc tggtcagaaa       60

ggcaccagcg cagaacaggt taccaatatt gataaaaccg tgaaacagct gatcgatgtg      120

gtggaaaaag gttatgaact ggttctgacc catggtaatg gtccgcaggt tggtgcgatt      180

ctgattcagc atgaaatggc aaaagaagca attccggcaa tgccgctgga tttttgtggt      240

gcagaaagcc agggtctgat tggttatatg ctgtgtcaga gcattcgtaa tgaatgtgca      300

ggtcgtggtc tgaaaaaaga agccgtttgt attgttaccc aggttgaagt tgatccgcat      360

gataaagcat ttcgtaatcc gaccaaaccg gtgggtccgt tttataccca ggccgaagca      420

gaacagaata tggcagaacg tggtgaacgt tggattgaag atgccggtcg tggttggcgt      480

aaagttgttc cgagtccgga accgaaagaa atcgttgaaa aagaagttat tcgcgcactg      540

gttgatcagg gcaccgttgt tattgcaagc ggtggtggtg gtattccggt ttgtcgtaat      600

gatcagggtc agctgtatgg tgttgaagca gtgattgata aagatctggc aggcgaacgt      660

ctggcactgg atgttggtgc agatattttt gttattctga ccgatgttag ccatgccatc      720

ctgcattata acacaccgca gcagaaaccg ctgaaaaaag ttaccctgga agaaatgaaa      780

aaatacatcg aagaaggcca ttttcgcgca ggtagcatgg gtccgaaagt tcgtgcagca      840

ctgaattttg ttaaaaaagg tggtggtcgt gcaattattg cccgtctgga tcaggttatt      900

ccggcactga aaggtgaagt tggcacccag attgaaagca ccctgtaa                   948


<210>  40
<211>  957
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Thermanaerovibrio 
       acidaminovorans carbamate kinase.

<400>  40
atggcagcaa ccaaagttgt tgttgcactg ggtggtaatg cactgcaaga agcaggcacc       60

cctccgaccg cagaagcaca gctggaagtt gtgaaaaaaa ccgcaaccta tctggccgaa      120

attagcaaac gtggttatga aatggcagtg gttcatggta atggtccgca ggttggtcgt      180

attgttctga gccaagaaat tgcagcacag gcaaatccgg aaacaccggc aatgccgttt      240

gatgtttgtg gtgcaatgag ccagggttat attggttatc agattcagca ggcactgcgt      300

gatgcactgc gtaatcgtaa tctgaatatt ccggttgtta ccctggttac ccaggttgtt      360

gtggatgcaa atgatccggc attcaaaaat ccgaccaaac cgattggtcc gttttatacc      420

gaagaagagg caaaaaaact gatggccgaa aaaggctatg tgatgaaaga agatgcaggt      480

cgcggttggc gtcgtgttgt tgccagtccg gaaccgaaaa aaatcaccga aatttcagca      540

gttaaacgcc tgtgggatac caccattgtt gttaccgcag gcggtggtgg tattccggtg      600

gttgaaaata tggatggtag cctgagcggt gttgcagcag ttattgataa agatctggca      660

gcagaaaaac tggccgaaga aattgaagcc gatattctgc tgattctgac cgaagttgat      720

aaagtgtgca tcaacttcaa aaaaccgaat cagcaagaac tgagccatat gaccgttgcc      780

gaagcaatca aatatatgga agaaggtcat tttgcaccgg gtagcatgct gccgaaagtt      840

atggcagccg ttaaatttgc acgtaccttt ccgggtaaaa aagccattat taccagcctg      900

tataaagcag ttgatgccct ggaaggtcgt gaaggcaccg ttgttaccat ggcataa         957


<210>  41
<211>  933
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Halothermothrix 
       orenii carbamate kinase

<400>  41
atggcacgta ttgttattgc actgggtggt aatgccctgg gtaaaacacc gctggaacag       60

cgtgaagcag tgaaaaatac cgcacagccg attgttgatc tgattgaaga tggccatgaa      120

attattctgg cacatggtaa tggtccgcag gttggtatga ttaatctggc atttgaaacc      180

gcagcacaga atgatgataa tgttccggaa atgccgtttc ctgaatgtgg tgcaatgagc      240

cagggttata ttggttatca tctgcagaat gccattcgca atgaactggt taatcgcggt      300

attaacaaaa gcattaccag cgttgttacc caggttctgg ttgataaaaa tgataacgca      360

tttgagcatc cgaccaaacc ggttggtagc ttttataccg aagatgaagc acgcaaactg      420

atcgaagaaa aaggttatcg tatggtggaa gatagcggtc gtggttatcg tcgtgttgtt      480

ccgagcccga ttccggttga tattgttgaa aaagagatca tcaaaaacct ggtcgaagat      540

ggcaatattg tgattgcgtg cggtggtggt ggcgttccgg ttgttgagga tgaagaaggt      600

ctgaaaggcg ttcctgcagt tattgataaa gattttgcca gcgaaaaaat ggccgaaatt      660

gttaatgccg atctgctggt tattctgacc gcagtggaaa aagttgcaat caactttggc      720

aaagaaaacg aagaatggct gagcgaaatg aatgttgaac tggcacagca gtattgtgat      780

gaaggtcatt ttgcaccggg tagcatgctg ccgaaagtta aagcagcaat gaaatttgca      840

agcagcaaag aaggtcgtaa agcactgatt accagcctgg aaaaagcaaa agaaggcctg      900

gcaggtaaaa ccggcaccct ggttaccgca taa                                   933


<210>  42
<211>  939
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Kosmotoga olearia 
       carbamate kinase

<400>  42
atgaaaaaag ccgttgttgc cattggtggt aatgcactga ataaaccggg tgaaaaaccg       60

agcgcagaag caatgaaaaa aaacctgctg ggcaccgtta aacatctggc agatctgatt      120

gaagatggtt atgatctggt tattacccat ggtaatggtc cgcaggttgg taatctgctg      180

gttcagcaag aaattgcaaa agataccctg cctccgtttc cgattgatgt taatgatgca      240

atgacccagg gtagcattgg ttatctgatt acccagaccc tgggcaatga actgaaaagc      300

cgtggcaccg aacgtcagat tgcatgtgtt ctgacccaga ttattgtgga tcgtaatgat      360

ccgggttttg agaatccgac caaaccggtg ggtccgtttt atgatgaaga aaccgcaaaa      420

aaactgcagg cagaaaaagg ctggatcatg aaagaagatg caggtcgcgg ttggcgtcgt      480

gttgttccga gcccgaaacc gctggatgtt gttgaaattg aagcaattaa agccctgacc      540

ggcaacgatt ttattctggt tgccggtggt ggtggcggta ttccggttgt taaaaaagat      600

gatggcaccc tggaaggtgt tgaagcagtt attgataaag atcgtgcaag cgcactgctg      660

gcacgtctgc tggatgcaga cctgtttctg attctgaccg cagttgatca tgcctatatc      720

aattttggta aaccggatca gaaagccctg gaacgtatta gcgttaatga agccaaaaaa      780

ctgatggatg aaggccattt tgccaaaggt agcatgtatc cgaaaattga aagcgccatt      840

gattttgttg aaagcaccgg taaagaagcc attattacca gcctggaaaa agtgaaagaa      900

gcgattcagg gtaaaagcgg cacccatatt accaaataa                             939


<210>  43
<211>  939
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Moorella 
       thermoacetica carbamate kinase

<400>  43
atggaacgtc tggcagttat tgcaattggt ggtaatagcc tgatcaaaaa caaaaaactg       60

atcagcctgt atgatcagct ggcaaccatg cgtgaaacct gtcgtaatat tgcagatatg      120

gttgaaaaag gctggaacgt tgttattacc catggtaatg gtccgcaggt tggttttctg      180

attcgtcgtg cagaactggc atgtggtgaa ctgccgctga ttccgctgga atttgcagtt      240

gcagataccc agggtgccat tggttatatg attcagcaga gcctgatgaa tgaatttcgt      300

cgtcgtggta ttaaacgtca ggccattaca attgttaccc aggttgttgt ggatcagaat      360

gatgaagcat ttcagaatcc gaccaaaccg attggtagct ttatgacccg tgaagaagca      420

cagcgtcacg ttgaagaaga tggttgggtt gttgttgaag atgcaggtcg tggttggcgt      480

cgtgttgttc cgagtccgga accgaaagca attgttgaaa ttcgtgcaat caaagacctg      540

atcgaagatg gctatattgt tatttgtacc ggtggtggtg gtattccggt tgttgaacat      600

gatggtaatc tgaaaggtgt tgcagccgtt gtggataaag ataatgcaag cgcactgctg      660

gccaatgaaa tcaaagcaga tgttctggtt attagcaccg gtgtgaataa agtggcaatt      720

aactttggtc gtccggatca gaaagaactg gatcgtctga ccattgccga agccgaagca      780

tatatggcag caggtcattt tccgcctggt aatatgggtc cgaaaattac agcactgctg      840

cgctatctga aaaatggtgg taaagaaggt attattacca gcccgaccga actggttgca      900

gcactggaag gtaaaagcgg cacccgtatt gttctgtaa                             939


<210>  44
<211>  310
<212>  PRT
<213>  Enterococcus faecalis

<400>  44

Met Gly Lys Lys Met Val Val Ala Leu Gly Gly Asn Ala Ile Leu Ser 
1               5                   10                  15      


Asn Asp Ala Ser Ala His Ala Gln Gln Gln Ala Leu Val Gln Thr Ser 
            20                  25                  30          


Ala Tyr Leu Val His Leu Ile Lys Gln Gly His Arg Leu Ile Val Ser 
        35                  40                  45              


His Gly Asn Gly Pro Gln Val Gly Asn Leu Leu Leu Gln Gln Gln Ala 
    50                  55                  60                  


Ala Asp Ser Glu Lys Asn Pro Ala Met Pro Leu Asp Thr Cys Val Ala 
65                  70                  75                  80  


Met Thr Gln Gly Ser Ile Gly Tyr Trp Leu Ser Asn Ala Leu Asn Gln 
                85                  90                  95      


Glu Leu Asn Lys Ala Gly Ile Lys Lys Gln Val Ala Thr Val Leu Thr 
            100                 105                 110         


Gln Val Val Val Asp Pro Ala Asp Glu Ala Phe Lys Asn Pro Thr Lys 
        115                 120                 125             


Pro Ile Gly Pro Phe Leu Thr Glu Ala Glu Ala Lys Glu Ala Met Gln 
    130                 135                 140                 


Ala Gly Ala Ile Phe Lys Glu Asp Ala Gly Arg Gly Trp Arg Lys Val 
145                 150                 155                 160 


Val Pro Ser Pro Lys Pro Ile Asp Ile His Glu Ala Glu Thr Ile Asn 
                165                 170                 175     


Thr Leu Ile Lys Asn Asp Ile Ile Thr Ile Ser Cys Gly Gly Gly Gly 
            180                 185                 190         


Ile Pro Val Val Gly Gln Glu Leu Lys Gly Val Glu Ala Val Ile Asp 
        195                 200                 205             


Lys Asp Phe Ala Ser Glu Lys Leu Ala Glu Leu Val Asp Ala Asp Ala 
    210                 215                 220                 


Leu Val Ile Leu Thr Gly Val Asp Tyr Val Cys Ile Asn Tyr Gly Lys 
225                 230                 235                 240 


Pro Asp Glu Lys Gln Leu Thr Asn Val Thr Val Ala Glu Leu Glu Glu 
                245                 250                 255     


Tyr Lys Gln Ala Gly His Phe Ala Pro Gly Ser Met Leu Pro Lys Ile 
            260                 265                 270         


Glu Ala Ala Ile Gln Phe Val Glu Ser Gln Pro Asn Lys Gln Ala Ile 
        275                 280                 285             


Ile Thr Ser Leu Glu Asn Leu Gly Ser Met Ser Gly Asp Glu Ile Val 
    290                 295                 300                 


Gly Thr Val Val Thr Lys 
305                 310 


<210>  45
<211>  315
<212>  PRT
<213>  Clostridium tetani

<400>  45

Met Asp Asn Lys Thr Ile Leu Val Ala Leu Gly Gly Asn Ala Ile Leu 
1               5                   10                  15      


Gln Pro Gly Gln Phe Ala Ser Tyr Glu Asn Gln Leu Asn Asn Val Lys 
            20                  25                  30          


Thr Ser Cys Arg Val Leu Ala Arg Leu Ile Lys Lys Gly Tyr Arg Leu 
        35                  40                  45              


Ile Ile Thr His Gly Asn Gly Pro Gln Val Gly Asn Ile Ile Arg Gln 
    50                  55                  60                  


Asn Glu Glu Ala Ser Ser Val Val Pro Pro Met Pro Met Asp Val Cys 
65                  70                  75                  80  


Ser Ala Glu Ser Gln Gly Phe Ile Gly Tyr Met Ile Gln Gln Ser Met 
                85                  90                  95      


Met Asn Glu Leu Val Asn Leu Gly Leu Glu Ile Pro Val Val Thr Phe 
            100                 105                 110         


Val Thr Arg Val Glu Val Tyr Glu Lys Asp Ser Ala Phe Lys Asn Pro 
        115                 120                 125             


Thr Lys Pro Ile Gly Met Phe Tyr Ser Glu Glu Gln Ala Lys Lys Leu 
    130                 135                 140                 


Met Glu Glu Lys Gln Trp Ile Leu Lys Pro Asp Ala Asn Arg Gly Trp 
145                 150                 155                 160 


Arg Arg Val Val Pro Ser Pro Lys Pro Ile Asn Ile Val Glu Thr Lys 
                165                 170                 175     


Ser Ile Arg Lys Leu Ile Asp Glu Gly Asn Ile Val Ile Ala Cys Gly 
            180                 185                 190         


Gly Gly Gly Ile Pro Val Met Arg Cys Glu Asp Asn Thr Tyr Lys Gly 
        195                 200                 205             


Val Glu Ala Val Ile Asp Lys Asp Arg Ser Gly Cys Lys Leu Ala Gln 
    210                 215                 220                 


Gln Ala Glu Ala Asp Met Phe Ile Ile Leu Thr Asp Val Glu Asn Ala 
225                 230                 235                 240 


Cys Ile Asn Tyr Gly Lys Glu Asn Gln Lys Ser Leu Gly Lys Val Lys 
                245                 250                 255     


Ile Glu Glu Leu Glu Gln Tyr Ile Glu Lys Gly Glu Phe Ser Lys Gly 
            260                 265                 270         


Ser Met Leu Pro Lys Val Glu Ser Ala Val Glu Phe Val Lys Arg Thr 
        275                 280                 285             


Asn Gly Ile Ser Ile Ile Cys Ala Leu Asp Lys Ala Glu Leu Ala Ile 
    290                 295                 300                 


Glu Gly Lys Ala Gly Thr Ile Ile Arg Lys Ala 
305                 310                 315 


<210>  46
<211>  930
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Enterococcus 
       faecalis carbamate kinase

<400>  46
atgggcaaaa aaatggttgt tgcactgggt ggtaatgcca ttctgagcaa tgatgcaagc       60

gcacatgcac agcagcaggc actggttcag accagcgcat atctggttca tctgattaaa      120

cagggtcatc gtctgattgt tagccatggt aatggtccgc aggttggtaa tctgcttctg      180

caacagcagg ctgcagatag cgaaaaaaat ccggcaatgc cgctggatac ctgtgttgca      240

atgacccagg gtagcattgg ttattggctg agcaatgcac tgaatcaaga actgaataaa      300

gccggtatca aaaaacaggt tgcaaccgtt ctgacccagg ttgttgttga tccggcagat      360

gaagcattca aaaatccgac caaaccgatt ggtccgtttc tgaccgaagc cgaagcaaaa      420

gaagcaatgc aggcaggcgc aatctttaaa gaagatgcag gtcgcggttg gcgtaaagtt      480

gttccgagcc cgaaaccgat tgatattcat gaagcagaaa ccattaatac cctgattaaa      540

aacgatatca ttaccattag ctgcggtggt ggtggtattc cggttgttgg ccaagaactg      600

aaaggcgttg aagcagttat tgataaagat tttgccagcg aaaaactggc cgaactggtt      660

gatgcagatg cactggttat tctgaccggt gttgattatg tgtgcattaa ctatggcaaa      720

ccggatgaaa aacagctgac caatgttacc gttgcagaac tggaagaata taaacaggca      780

ggtcattttg caccgggtag catgctgccg aaaattgaag cagcaattca gtttgttgaa      840

agccagccga ataaacaggc aattattacc agcctggaaa atctgggtag catgagcggt      900

gatgaaattg ttggcaccgt tgtgaccaaa                                       930


<210>  47
<211>  945
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized nucleotide sequence encoding Clostridium tetani 
       carbamate kinase

<400>  47
atggataaca aaaccattct ggttgccctg ggtggtaatg caattctgca gcctggtcag       60

tttgcaagct atgaaaatca gctgaataat gtgaaaacca gctgtcgtgt tctggcacgt      120

ctgatcaaaa aaggttatcg cctgattatt acccatggta atggtccgca ggttggtaac      180

attattcgtc agaatgaaga agccagcagc gttgttcctc cgatgccgat ggatgtttgt      240

agcgcagaaa gccagggttt tattggttat atgattcagc agagcatgat gaatgaactg      300

gttaatctgg gtctggaaat tccggttgtt acctttgtta cccgtgttga agtgtatgaa      360

aaagatagcg ccttcaaaaa tccgaccaaa ccgattggta tgttttatag cgaagaacag      420

gccaaaaaac tgatggaaga aaaacagtgg attctgaaac cggatgcaaa tcgtggttgg      480

cgtcgtgttg ttccgagccc gaaaccgatt aacattgttg aaaccaaaag cattcgcaaa      540

ctgattgatg aaggcaatat tgttattgcc tgtggtggtg gtggtattcc ggttatgcgt      600

tgtgaagata acacctataa aggtgtggaa gccgtgattg ataaagatcg tagcggttgt      660

aaactggcac agcaggccga agcagatatg tttatcattc tgaccgatgt ggaaaatgcc      720

tgcattaact atggcaaaga aaatcagaaa agcctgggca aagtgaaaat tgaagaactg      780

gaacagtata ttgaaaaagg cgaatttagc aaaggtagca tgctgccgaa agttgaaagc      840

gcagttgaat ttgttaaacg caccaatggc attagcatta tttgtgcact ggataaagca      900

gaactggcca ttgaaggtaa agcaggcacc attattcgta aagcc                      945


<210>  48
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  48
gtggtttcca tgggtaagag ggtagtgatt gc                                     32


<210>  49
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  49
gcattcgcta agctgggtct tctaaagttc ctcagg                                 36

